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Journal / Biochemistry / PeptiQuick, et et-trins inkorporering af membran…
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Biochemistry
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JoVE Journal Biochemistry
PeptiQuick, et et-trins inkorporering af membran proteiner i Biotinylerede Peptiskiver for strømlinede protein bindende assays
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PeptiQuick, et et-trins inkorporering af membran proteiner i Biotinylerede Peptiskiver for strømlinede protein bindende assays

Article DOI: 10.3791/60661-v • 15:04 min • November 2nd, 2019
November 2nd, 2019 •
1, 2, 1
1Department of Biochemistry, University of British Columbia, 2School of Biochemistry, University of Bristol

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Vi præsenterer en metode, der kombinerer membran protein rensning og rekonstitution i peptidiscs i et enkelt kromatografisk trin. Biotinylerede stilladser anvendes til direkte overflade fastgørelse og måling af protein-ligand interaktioner via biolayer interferometri.

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Biokemi membran mimetika nanoskiver membran proteiner receptorer kanaler transportører biosensor lipiderne colicin biotin-streptavidin protein-protein interaktioner
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