In JoVE (2)
Other Publications (0)
Articles by Yu Liu in JoVE
Modeling Myotonic Dystrophy 1 in C2C12 Myoblast Cells Rui Liang*1, Wei Dong*1,3, Xiaopeng Shen1, Xiaoping Peng1,3, Angie G. Aceves1,2, Yu Liu1 1Department of Biology and Biochemistry, University of Houston, 2Department of Economics, University of Houston, 3Department of Cardiology, The First Affiliated Hospital of Nanchang University In this protocol, we present the procedures in establishing myotonic dystrophy 1 myoblast models, including optimized C2C12 cell maintenance, gene transfection/transduction, and myocyte differentiation.
Pancreatic Islet Embedding for Paraffin Sections Yahui Kong1, Pantea Ebrahimpour1,2, Yu Liu3, Chaoxing Yang1, Laura C. Alonso1 1Diabetes Center of Excellence, UMass Medical School, 2Department of Medicine, Saint Vincent Hospital, 3Department of Pathology, Morphology Core, UMass Medical School Ex vivo pancreatic islet studies are important for diabetes research. Existing techniques to study cultured islets in their native 3-dimensional architecture are time consuming, inefficient, and infrequently used. This work describes a new, simple, and efficient method for generating high-quality paraffin sections of whole cultured islets.