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Rapid PCR Thermocycling using Microscale Thermal Convection
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Rapid PCR Thermocycling using Microscale Thermal Convection

Article DOI: 10.3791/2366-v • 09:02 min • March 5th, 2011
March 5th, 2011 •
1, 2, 3
1Department of Mechanical Engineering, Texas A&M University, 2Department of Mechanical Engineering and Department of Nuclear Engineering, Texas A&M University, 3Department of Chemical Engineering, Texas A&M University

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We describe a novel method to perform DNA replication via the polymerase chain reaction (PCR). Thermal convection is harnessed to continuously shuttle reagents between denaturing, annealing, and extension conditions by maintaining opposing surfaces of the reactor at constant temperature. This inherently simple design promises to make rapid PCR more accessible.

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PCR Thermocycling Rapid PCR Microscale Thermal Convection Polymerase Chain Reaction Thermal Convection PCR Thermocycling Hardware Thermoelectric Heaters Reaction Speed Electrical Power Portable Format Convective Flows Buoyancy Driven Instability Spatial Temperature Gradient Reactor Geometry Denaturing Stage Annealing Stage Extension Stage
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