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人鼻粘膜上皮细胞在气液界面培养

Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface

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生物学

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JoVE 杂志生物学

Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface

人鼻粘膜上皮细胞在气液界面培养

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36,836 Views

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10:38 min

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October 08, 2013

DOI:

10.3791/50646-v

DOI:

10.3791/50646-v

•

10:38 min

October 08, 2013

•

25 Views

Loretta Müller¹, Luisa E. Brighton¹, Johnny L. Carson^1,2, William A. Fischer II^1,3, Ilona Jaspers^1,2,4

¹Center for Environmental Medicine, Asthma, and Lung Biology,The University of North Carolina at Chapel Hill, ²Department of Pediatrics,The University of North Carolina at Chapel Hill, ³Pulmonary Diseases and Critical Care,The University of North Carolina at Chapel Hill, ⁴Curriculum in Toxicology,The University of North Carolina at Chapel Hill

Summary

鼻粘膜上皮细胞，通过志愿者的浅表刮活检，是扩大和转移到组织培养插入。一旦达到融合，细胞生长在空气液体界面，产生纤毛和非纤毛细胞的培养。分化鼻粘膜上皮细胞培养提供可行的实验模型为研究呼吸道黏膜。

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