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Culture of Adult Transgenic Zebrafish Retinal Explants for Live-cell Imaging by Multiphoton Microscopy
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发育生物学
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JoVE 杂志 发育生物学
Culture of Adult Transgenic Zebrafish Retinal Explants for Live-cell Imaging by Multiphoton Microscopy

Culture of Adult Transgenic Zebrafish Retinal Explants for Live-cell Imaging by Multiphoton Microscopy

DOI:
10.3791/55335-v

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11:55 min

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February 24, 2017

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, , ,
1Department of Biological Sciences,University of Notre Dame, 2Department of Neurosurgery,Mayo Clinic

Chapters

  • 00:05Title
  • 01:11Isolation and Culturing of Retinal Explants
  • 03:30Multiphoton Microscopy
  • 07:52Analysis of Velocity
  • 09:34Results: Analysis of Apical and Basal Migration Velocities
  • 10:55Conclusion

Summary

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Zebrafish retinal regeneration has mostly been studied using fixed retinas. However, dynamic processes such as interkinetic nuclear migration occur during the regenerative response and require live-cell imaging to investigate the underlying mechanisms. Here, we describe culture and imaging conditions to monitor Interkinetic Nuclear Migration (INM) in real-time using multiphoton microscopy.

Tags

Retinal ExplantLive-cell ImagingMultiphoton MicroscopyZebrafishInterkinetic Nuclear MigrationRetinal RegenerationMuller GliaMicroglial BehaviorPhagocytosisAgaroseFluoroDish

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