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Immunology and Infection

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JoVE Journal Immunology and Infection
Estudando infecção por Cryptosporidium em sistemas de cultura Organóide humano derivado do tecido 3D por microinjeção
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Estudando infecção por Cryptosporidium em sistemas de cultura Organóide humano derivado do tecido 3D por microinjeção

Article DOI: 10.3791/59610-v • 05:31 min • September 14th, 2019
September 14th, 2019 •
1, 1,3, 2
1Hubrecht Institute, Oncode Institute, Royal Netherlands Academy of Arts and Sciences (KNAW), UMC Utrecht, 2Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, 3Janssen Pharmaceutica

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Nós descrevemos protocolos para preparar ooocysts e purificar esporozoítos para estudar a infecção de organóides intestinais e da via aérea humanos pelo parvum de Cryptosporidium. Nós demonstramos os procedimentos para o microinjection dos parasita no lúmen organoid intestinal e na imunocoloração dos organoids. Finalmente, nós descrevemos o isolamento de ooocysts gerados dos organoids.

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Imunologia e infecção Cryptosporidium organóides microinjeção hospedeiro-micróbio intestino pulmão criptosporidiose esporozoites oocistos
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