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Bioengineering

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JoVE Journal Bioengineering
Samtidig kvantifisering av utvalgte kynureniner analysert av flytende kromatografi-massespektrometri i medium samlet fra kreftcellekulturer
Environmental Dynamic Mechanical Analysis to Predict the Softening Behavior of Neural Implants
Journal (Bioengineering)
Environmental Dynamic Mechanical Analysis to Predict the Softening Behavior of Neural Implants
An Improved Method for the Preparation of Type I Collagen From Skin
Journal (Bioengineering)
An Improved Method for the Preparation of Type I Collagen From Skin
 
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Samtidig kvantifisering av utvalgte kynureniner analysert av flytende kromatografi-massespektrometri i medium samlet fra kreftcellekulturer

Article DOI: 10.3791/61031-v • 10:38 min • May 9th, 2020
May 9th, 2020 •
1, 1, 1
1Laboratory of Separation and Spectroscopic Method Applications, Centre for Interdisciplinary Research, The John Paul II Catholic University of Lublin

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Beskrevet her er en protokoll for bestemmelse av fire forskjellige tryptofan metabolitter generert i kynurenin banen (kynurenine, 3-hydroxykynurenine, xanthurenic syre, 3-hydroksyantranisyre) i mediet samlet inn fra kreftcellekulturer analysert av flytende kromatografi kombinert med en enkelt quadrupole massespektrometri.

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Bioengineering Utgave 159 flytende kromatografi massespektrometri tryptofan metabolitter kynurenine 3-hydroxykynurenine xanthurenic syre 3-hydroksyantranisyre kreftceller prøve forberedelse
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