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Journal / Neuroscience / 在多地址基因组集成颜色 （MAGIC） 标记的 Utero 电冲中，…
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Neuroscience

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JoVE Journal Neuroscience
在多地址基因组集成颜色 （MAGIC） 标记的 Utero 电冲中， 以个性化皮质小鼠星形细胞
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在多地址基因组集成颜色 （MAGIC） 标记的 Utero 电冲中， 以个性化皮质小鼠星形细胞

Article DOI: 10.3791/61110-v • 06:47 min • May 21st, 2020
May 21st, 2020 •
*1, *2, 1, 3, 4, 2, 1, 4, 3, 2, 1
1Université de Montpellier, INSERM, Institut des Neurosciences de Montpellier, 2Sorbonne Université, INSERM, CNRS, Institut de la Vision, 3Université Paris-Saclay, CEA, CNRS, MIRCen, Laboratoire des Maladies Neurodégénératives, 4Laboratory for Optics and Biosciences, Ecole polytechnique, CNRS, INSERM, IP Paris
* These authors contributed equally

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Summary
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星形细胞均匀地平铺大脑皮层，使得对其复杂形态的分析在细胞水平上具有挑战性。此处提供的协议使用基于子宫电镀的多色标签，挑出皮质星形细胞，并使用用户友好的图像分析管道分析其体积和形态。

Tags

神经科学，第159期，大脑皮层，星形细胞，发育，细胞体积，形态学，转基因，电化，基因转移技术，显微镜
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