JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
Journal
/
Neuroscience
/
在子宫内
电途径研究神经元亚群的兴奋性和单细胞连接
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Neuroscience
In Utero
Electroporation Approaches to Study the Excitability of Neuronal Subpopulations and Single-cell Connectivity
Please note that all translations are automatically generated.
Click here for the English version.
在子宫内
电途径研究神经元亚群的兴奋性和单细胞连接
DOI:
10.3791/55139-v
•
10:49 min
•
February 15, 2017
•
Carlos G. Briz
,
Marta Navarrete
,
José A. Esteban
,
Marta Nieto
1
Department for Molecular and Cellular Biology
,
Centro Nacional de Biotecnología (CNB-CSIC)
,
2
Molecular Neurobiology Department, Centro de Biología Molecular “Severo Ochoa”
,
Consejo Superior de Investigaciones Científicas (CSIC-UAM)
Chapters
00:05
Title
00:48
Injection of DNA and Electroporation
01:52
Sectioning and Staining of Brain Tissue
04:17
Imaging and Analysis
05:42
Electrophysiology
08:29
Results: Representative Firing Pattern of a CAG-GFP Electroporated Pyramidal Neuron
09:39
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
这份手稿规定,
在子宫内
电(IUE)用来描述在单细胞水平与荧光标记的神经元兴奋性神经元的结构连接协议。组织学是用于表征树突和轴突突起。在急性切片全细胞记录被用来研究兴奋性。
Tags
In Utero Electroporation
Neuronal Subpopulations
Single-cell Connectivity
Developmental Neurobiology
Cognitive Disorders
Sparse Population Of Neurons
Dendrites
Patch Clamp Study
Lateral Ventricle
Cryoprotection
Cryosectioning
Immunohistochemistry
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
在子宫内
的电穿孔其次原发性神经文化为研究基因的功能在皮层神经元的一个子集
在子宫内电穿孔:鼠标控制时空基因Transefection
神经元形态的研究方法:<em>体外</em> RNAi技术在胚胎小鼠大脑皮质的电穿孔
可视化和遗传操作在小鼠大脑皮层和使用海马树突和棘<em>在子宫内</em>电穿孔
外用转基因大鼠通过代<em>在子宫内</em>电穿孔和<em>在体内</em>生物发光筛选
遗传操纵鼠标的发展下丘脑通过<em>在子宫内</em>电穿孔
谷氨酸和GABA快速微离子导入:一个有用的工具,调查突触整合
小脑颗粒神经元的遗传操作<em>体外</em>和<em>在体内</em>为研究神经元形态和迁移
等电脑国家的诱导研究内源性突触活动对神经元兴奋性影响<em>在体内</em
分析树突状形态的列和图层
Read Article