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Isolamento das células CD8+ T residentes em rim do rato para análise de citometria de fluxo

Article DOI: 10.3791/61559-v • 06:07 min • June 27th, 2020
June 27th, 2020 •
1,2, 1, 1
1Department of Microbiology, Immunology and Molecular Genetics, School of Medicine, University of Texas Health Science Center at San Antonio, 2Department of Dermatology, Hunan Key Laboratory of Medical Epigenomics, The Second Xiangya Hospital, Central South University

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Após a infecção viral, o rim abriga um número relativamente grande de células CD8+ T e oferece uma oportunidade de estudar células TRM não mucosas. Aqui, descrevemos um protocolo para isolar linfócitos renais de camundongos para análise de citometria de fluxo.

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Este mês em JoVE Edição 160 CD8 residente de rim LCMV citometria de fluxo rato rotulagem intravascular
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