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Assessing Functional Metrics of Skeletal Muscle Health in Human Skeletal Muscle Microtissues

Article DOI: 10.3791/62307-v • 09:30 min • February 18th, 2021
February 18th, 2021 •
1,2, 1,2, 1,2, 1,2,3
1Institute of Biomedical Engineering, University of Toronto, 2Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 3Department of Cell and Systems Biology, University of Toronto

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This manuscript describes a detailed protocol to produce arrays of 3D human skeletal muscle microtissues and minimally invasive downstream in situ assays of function, including contractile force and calcium handling analyses.

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Functional Metrics Skeletal Muscle Health Skeletal Muscle Microtissues 3D Culture Muscle Biology Disease Modeling Candidate Molecule Testing Contractile Force Calcium Transients Longitudinal Studies Myotactic Culture Pluronic F-127 Solution Basement Membrane Extract Thrombin Saline Solution Fibrinogen
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