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Journal / Immunology and Infection / Studio dello sviluppo delle cellule dendritiche…
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Immunology and Infection

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JoVE Journal Immunology and Infection
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Studio dello sviluppo delle cellule dendritiche mediante knockdown genico mediato da RNA a forcina corta in una linea cellulare ematopoietica e progenitrice in vitro

Article DOI: 10.3791/62730-v • 06:12 min • March 7th, 2022
March 7th, 2022 •
1, 2, 1
1Graduate Institute of Immunology, National Taiwan University College of Medicine, 2Department of Pathology, Division of Microbiology and Immunology, University of Utah

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Qui forniamo un protocollo per lo screening di potenziali fattori di trascrizione coinvolti nello sviluppo di cellule dendritiche (DC) utilizzando la trasduzione lentivirale di shRNA per ottenere linee cellulari di knockdown stabili per la differenziazione DC in vitro .

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Immunologia e infezione Numero 181 sviluppo cellule dendritiche knockdown progenitori ematopoiesi fattori di trascrizione
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