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Journal
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Immunology and Infection
/
短发夹RNA介导的基因敲低在造血干和祖细胞系
体外
发育的树突状细胞发育研究
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line
In vitro
Please note that all translations are automatically generated.
Click here for the English version.
短发夹RNA介导的基因敲低在造血干和祖细胞系
体外
发育的树突状细胞发育研究
DOI:
10.3791/62730-v
•
06:12 min
•
March 07, 2022
•
Yu-Ling Hsiao
,
Hans Häcker
,
Chien-Kuo Lee
1
Graduate Institute of Immunology
,
National Taiwan University College of Medicine
,
2
Department of Pathology, Division of Microbiology and Immunology
,
University of Utah
Chapters
00:04
Introduction
00:43
Preparation of Immortalized HematopoieticStem and Progenitor Cell (iHSPC) Lines and Lentiviral Transduction
02:08
In Vitro Differentiation of the Stable Knockdown iHSPC Cell Lines
03:05
Flow Cytometric Analysis of the Differentiated Dendritic Cells
04:01
Results: Dendritic Cell Differentiation Following Short Hairpin RNA-Mediated
Tcf4
and
Id2
Knockdown
05:33
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提供了一种方案,用于筛选参与树突状细胞(DC)发展的潜在转录因子,使用shRNA的慢病毒转导来获得稳定的敲低细胞系,用于
体外
DC分化。
Tags
Dendritic Cell Development
Short Hairpin RNA
Gene Knockdown
Hematopoietic Stem And Progenitor Cell Line
In Vitro
Transcription Factors
FLT3 Ligand
Beta Estradiol
Lentiviral Transduction
Puromycin
LacZ
Tcf4
Id2
Flow Cytometry
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