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Journal / Immunology and Infection / Estudio del desarrollo de células dendríticas…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Estudio del desarrollo de células dendríticas mediante la eliminación de genes mediados por ARN de horquilla corta en una línea de células madre y progenitoras hematopoyéticas in vitro
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Estudio del desarrollo de células dendríticas mediante la eliminación de genes mediados por ARN de horquilla corta en una línea de células madre y progenitoras hematopoyéticas in vitro

Article DOI: 10.3791/62730-v • 06:12 min • March 7th, 2022
March 7th, 2022 •
1, 2, 1
1Graduate Institute of Immunology, National Taiwan University College of Medicine, 2Department of Pathology, Division of Microbiology and Immunology, University of Utah

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Aquí proporcionamos un protocolo para el cribado de posibles factores de transcripción implicados en el desarrollo de células dendríticas (DC) mediante la transducción lentiviral de shRNA para obtener líneas celulares de derribo estables para la diferenciación in vitro de DC.

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Inmunología e Infección Número 181 desarrollo célula dendrítica derribo progenitores hematopoyesis factores de transcripción
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