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Journal / Immunology and Infection / Undersøgelse af dendritisk celleudvikling af…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Undersøgelse af dendritisk celleudvikling af short hairpin RNA-medieret gen knockdown i en hæmatopoietic stilk og stamfader Cell Line In vitro
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Flow Cytometry-based Assay for the Monitoring of NK Cell Functions
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Undersøgelse af dendritisk celleudvikling af short hairpin RNA-medieret gen knockdown i en hæmatopoietic stilk og stamfader Cell Line In vitro

Article DOI: 10.3791/62730-v • 06:12 min • March 7th, 2022
March 7th, 2022 •
1, 2, 1
1Graduate Institute of Immunology, National Taiwan University College of Medicine, 2Department of Pathology, Division of Microbiology and Immunology, University of Utah

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Her leverer vi en protokol til screening af potentielle transskriptionsfaktorer, der er involveret i udviklingen af dendritiske celle (DC) ved hjælp af lentiviral transduktion af shRNA for at opnå stabile knockdown cellelinjer til in vitro DC differentiering.

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Immunologi og infektion udvikling dendritisk celle knockdown forfædre hæmatopoiesis transskription faktorer
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