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Journal / Immunology and Infection / Estudo do Desenvolvimento de Células Dendríticas…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Estudo do Desenvolvimento de Células Dendríticas por Short Hairpin RNA-Mediado Gene Knockdown em uma linha de célula hematopoiética e progenitora in vitro
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Estudo do Desenvolvimento de Células Dendríticas por Short Hairpin RNA-Mediado Gene Knockdown em uma linha de célula hematopoiética e progenitora in vitro

Article DOI: 10.3791/62730-v • 06:12 min • March 7th, 2022
March 7th, 2022 •
1, 2, 1
1Graduate Institute of Immunology, National Taiwan University College of Medicine, 2Department of Pathology, Division of Microbiology and Immunology, University of Utah

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Aqui fornecemos um protocolo para triagem de potenciais fatores de transcrição envolvidos no desenvolvimento de células dendríticas (DC) usando transdução lentiviral de shRNA para obter linhas celulares de knockdown estáveis para diferenciação in vitro DC.

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Imunologia e Infecção Problema 181 desenvolvimento célula dendrítica knockdown progenitores hematopoiese fatores de transcrição
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