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Immunology and Infection

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Canine Intestinal Organoids in a Dual-Chamber Permeable Support System

Article DOI: 10.3791/63612-v • 11:11 min • March 2nd, 2022
March 2nd, 2022 •
1, 1, 2, 3, 2, 3, 4, 5, 2, 2,3, 1,3
1Department of Biomedical Sciences, College of Veterinary Medicine, Iowa State University, 2Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, 33D Health Solutions Inc., 4Office of New Animal Drug Evaluation, Center for Veterinary Medicine, Food and Drug Administration, 5Division of Applied Regulatory Science, Office of Clinical Pharmacology, Office of Translational Sciences, Center for Drug Evaluation and Research, Food and Drug Administration

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Here, we present a protocol describing the culture of canine intestinal organoids in a dual-chamber, permeable support system. Organoid seeding in the permeable supports, the monolayer maintenance, and subsequent drug permeability experiments are described.

Tags

Canine Intestinal Organoids Dual-chamber Permeable Support System In Vitro Tool Oral Permeability Therapeutic Drug Candidates Traditional Two-dimensional (2D) Cell Lines Limitations Tight Junctions Cell Differentiation Nuclear Receptors Caco-2 Model MDCK Model Translational Model Gastrointestinal Anatomy Intestinal Microflora In Vivo Drug Permeability Characteristics Canine Intestinal Organoid System Three-dimensional (3D) Self-assembled Epithelial Structures Adult Stem Cells Permeable Support Seeding Protocol
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