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Human saphenøs veneendotelcelleisolering og eksponering for kontrollerede niveauer af forskydningsstress og strækning

Article DOI: 10.3791/65122-v • 09:10 min • April 21st, 2023
April 21st, 2023 •
*1,2, *1, 1, 1, 1, 1, 1
1Instituto do Coraçao (InCor), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, 2Cardiology Research Group, Faculty VI Medicine and Health Sciences, Carl von Ossietzky University of Oldenburg
* These authors contributed equally

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Vi beskriver en protokol til isolering og dyrkning af humane saphenøse veneendotelceller (hSVEC'er). Vi leverer også detaljerede metoder til at producere forskydningsspænding og stræk for at studere mekanisk belastning i hSVEC'er.

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Denne måned i JoVE nummer 194
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