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JoVE Journal Medicine
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Isolering av endotelceller i humane safenøse vener og eksponering for kontrollerte nivåer av skjærspenning og strekk

Article DOI: 10.3791/65122-v • 09:10 min • April 21st, 2023
April 21st, 2023 •
*1,2, *1, 1, 1, 1, 1, 1
1Instituto do Coraçao (InCor), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, 2Cardiology Research Group, Faculty VI Medicine and Health Sciences, Carl von Ossietzky University of Oldenburg
* These authors contributed equally

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Vi beskriver en protokoll for å isolere og dyrke humane endotelceller i saphenøse vene (hSVECs). Vi tilbyr også detaljerte metoder for å produsere skjærspenning og strekk for å studere mekanisk belastning i hSVEC.

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