JoVE Journal
Immunology and Infection
Immunology and Infection
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
In vitro analysis of class switch recombination in mice is challenging due to cytophilic IgE molecules bound to Fc receptors on the surface of B cells. We describe a method for IgE detection using trypsin-mediated cleavage of surface-bound IgE prior to fixation and permeabilization for cytoplasmic fluorescence staining.