Journal
/
Genética
/
Preparation of rAAV9 to Overexpress or Knockdown Genes in Mouse Hearts
JoVE Revista
Genética
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Genética
Preparation of rAAV9 to Overexpress or Knockdown Genes in Mouse Hearts

Preparation of rAAV9 to Overexpress or Knockdown Genes in Mouse Hearts

DOI:
10.3791/54787-v

•

11:11 min

•

December 17, 2016

•

, , , , , ,
1Department of Cardiology,Boston Children’s Hospital, 2Department of Pediatrics,Harvard Medical School, 3Department of Genetics,Harvard Medical School, 4Howard Hughes Medical Institute

Capítulos

  • 00:05Título
  • 00:57Transfection of HEK293 with rAAV9 Plasmids
  • 02:14Harvest of Transfected HEK293 Cells and Purification of rAAV9 Vectors
  • 07:04rAAV9 Injections into Neonatal Mice
  • 08:10Results: rAAV9 Overexpression and Gene Knockdown in Mouse Hearts
  • 10:18Conclusion

Summary

Traducción Automática

Traducción Automática

In this manuscript, a method to prepare recombinant adeno-associated virus 9 (rAAV9) vectors to manipulate gene expression in the mouse heart is described.

Tags

RAAV9Gene ExpressionMouse HeartIn VivoGene FunctionCardiologyMolecular GeneticsGene ManipulationHEK293 CellsTransfectionPEICell CultureCell HarvestingCell LysisIodixanol GradientVirus Purification

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

The Use of Induced Somatic Sector Analysis (ISSA) for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast

Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay

Simultaneous Video-EEG-ECG Monitoring to Identify Neurocardiac Dysfunction in Mouse Models of Epilepsy

qKAT: Quantitative Semi-automated Typing of Killer-cell Immunoglobulin-like Receptor Genes

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster

Modulation of Tau Subcellular Localization as a Tool to Investigate the Expression of Disease-related Genes

Read Article