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Biochemistry

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Analysis of AtHIRD11 Intrinsic Disorder and Binding Towards Metal Ions by Capillary Gel Electrophoresis and Affinity Capillary Electrophoresis

Article DOI: 10.3791/57749-v • 07:54 min • August 22nd, 2018
August 22nd, 2018 •
1, 1, 1, 1
1Institute of Medicinal and Pharmaceutical Chemistry, TU Braunschweig

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This protocol combines the characterization of a protein sample by capillary gel electrophoresis and a fast-binding screening for charged ligands by affinity capillary electrophoresis. It is recommended for proteins with a flexible structure, such as intrinsically disordered proteins, to determine any differences in binding for different conformers.

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AtHIRD11 Intrinsic Disorder Binding Metal Ions Capillary Gel Electrophoresis Affinity Capillary Electrophoresis Conformational Changes Equipment Results Procedure Glass Cutter Glass Plate Fused Silica Capillary Polyamide External Coating Inner Diameter Detection Window CGE Experiment ACE Experiment Blowtorch Ethanol Syringe Calcium Chloride Solution PVDF Filter
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