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A Simple and Effective Method to Consistently Isolate Mouse Cardiomyocytes
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JoVE Journal Medicine
A Simple and Effective Method to Consistently Isolate Mouse Cardiomyocytes

A Simple and Effective Method to Consistently Isolate Mouse Cardiomyocytes

DOI:
10.3791/63056-v

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06:25 min

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November 11, 2022

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, , ,
1Department of Physiology and Cell Biology,The Ohio State University

Chapters

  • 00:04Introduction
  • 00:45Manifold Preparation and Cardiomyocyte Isolation Procedure
  • 05:24Results: Isolation of High‐Quality Mouse Cardiomyocytes
  • 05:55Conclusion

Summary

Automatic Translation

Automatic Translation

The gold standard in cardiology for cellular and molecular functional experiments are cardiomyocytes. This article describes adaptations to the non-Langendorff technique to isolate mouse cardiomyocytes.

Tags

Cardiomyocyte IsolationMouse HeartLangendorff ApparatusIschemic TimeIn Vivo PerfusionDigestion BufferTemperature-controlled ManifoldSternumAortaVentricleTweezersHemostatGraduate StudentLaboratory Technique

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