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Developmental Biology

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Generering av Induced Pluripotent stamceller fra Frozen Buffy Coats bruker Non-integrere episomal Plasmider

Article DOI: 10.3791/52885-v • 10:52 min • June 5th, 2015
June 5th, 2015 •
*1, *1, 1, 1, 2, 2, 3, 1, 1, 1
1Center for Biomedicine, European Academy Bozen/Bolzano (EURAC), 2Laboratory of Medical Genetics, Fondazione IRCCS Ca´ Granda, Ospedale Maggiore Policlinico, 3Del E. Webb Center for Neuroscience, Aging & Stem Cell Research, Sanford-Burnham Medical Research Institute
* These authors contributed equally

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Induserte pluripotente stamceller (iPSCs) representerer en kilde til pasientspesifikke vev for kliniske applikasjoner og grunnforskning. Her presenterer vi en detaljert protokoll for å omprogrammere humane perifere mononukleære blodceller (PBMNCs) fra fryste buffy coats inn virusfritt iPSCs med ikke-integrerende episomale plasmider.

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Developmental Biology Stem cellebiologi cellebiologi molekylærbiologi induserte pluripotente stamceller perifere mononukleære blodceller omprogrammering episomale plasmider.
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