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Journal / Genetics / 单细胞基因表达利用多重RT-qPCR的表征罕见的淋巴人群的异质性…
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Genetics

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JoVE Journal Genetics
单细胞基因表达利用多重RT-qPCR的表征罕见的淋巴人群的异质性
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单细胞基因表达利用多重RT-qPCR的表征罕见的淋巴人群的异质性

Article DOI: 10.3791/54858-v • 10:23 min • January 19th, 2017
January 19th, 2017 •
1, 1, 2, 1, 1
1Unit for Lymphopoieseis, Immunology Department, INSERM U1223, University Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Institut Pasteur, 2Center for Translational Science, Institut Pasteur, INSERM UMS20

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这个协议描述如何评估在克隆水平大阵列的基因的表达。单细胞RT-qPCR的产生与数百个样品和基因的敏感性较强高度可靠的结果。

Tags

遗传学，第119，单细胞，基因表达，表达模式，细胞的异质性，微流控，先天淋巴样细胞（ILC）
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