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Journal / Developmental Biology / Eine geschichtete Montagemethode für die…
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Developmental Biology

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JoVE Journal Developmental Biology
Eine geschichtete Montagemethode für die verlängerte Zeitraffer-Konfokalmikroskopie ganzer Zebrafisch-Embryonen
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Eine geschichtete Montagemethode für die verlängerte Zeitraffer-Konfokalmikroskopie ganzer Zebrafisch-Embryonen

Article DOI: 10.3791/60321-v • 08:55 min • January 14th, 2020
January 14th, 2020 •
1, 2, 2, 3,4, 1,4, 5
1Computational Biomedicine Lab, Texas Institute of Measurement Evaluation and Statistics, University of Houston, 2Institute of Biosciences and Technology, Texas A&M Health Science Center, 3Department of Biology and Biochemistry, Center for Nuclear Receptors and Cell Signaling, University of Houston, 4Department of Biosciences and Nutrition, Novum, Karolinska Institutet, 5Department of Intelligent Systems Engineering, Indiana University

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Dieser Artikel beschreibt eine Methode zur Montage zerbrechlicher Zebrafischembryonen für eine längere konfokale Zeitraffermikroskopie. Diese kostengünstige Methode ist einfach mit normalen Glasbodenmikroskopie-Geschirr für die Bildgebung auf jedem invertierten Mikroskop durchzuführen. Die Montage erfolgt in Agaroseschichten in unterschiedlichen Konzentrationen.

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Entwicklungsbiologie Ausgabe 155 Zebrafisch Embryo konfokale Mikroskopie Zeitraffer Montage transgen
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