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Isolamento de núcleos de tumores cerebrais congelados frescos para RNA-seq de núcleo único e ATAC-seq

Article DOI: 10.3791/61542-v • 06:22 min • August 25th, 2020
August 25th, 2020 •
1, 2, 1, 1,3, 4, 2, 1
1Neurology Clinic and National Center for Tumor Diseases, University Hospital Heidelberg, 2Bioinformatics and Omics Data Analytics, German Cancer Research Center (DKFZ), 3Clinical Cooperation Unit Neurooncology, German Cancer Research Center (DKFZ), 4Division of Experimental Neurosurgery, Department of Neurosurgery, University of Heidelberg, INF 400, Heidelberg, Germany

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A heterogeneidade intra-tumoral é uma característica inerente aos tumores, incluindo gliomas. Desenvolvemos um protocolo simples e eficiente que utiliza uma combinação de buffers e centrífugas gradientes para isolar núcleos únicos de tecidos glioma congelados frescos para estudos de sequenciamento de núcleo único RNA e ATAC.

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Pesquisa do Câncer Questão 162 Isolamento dos Núcleos snRNA-seq snATAC-seq 10x Genômica Glioma
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