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Crystallization of Membrane Proteins in Lipidic Mesophases

Article DOI: 10.3791/2501-v • 11:53 min • March 28th, 2011
March 28th, 2011 •
1, 1
1Molecular Biology, The Scripps Research Institute

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The protocols describe the essential steps for obtaining diffraction quality crystals of a membrane protein starting from reconstitution of the protein in a lipidic cubic phase (LCP), finding initial conditions with LCP-FRAP pre-crystallization assays, setting up LCP crystallization trials and harvesting crystals.

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Crystallization Membrane Proteins Lipidic Mesophases Signal Transduction Transport Energy Transformations Malfunctions Diseases Structural Understanding Functional Understanding Solubilization Diffraction Quality Crystals In Meso Crystallization Native-like Membrane Environment Solvent Content Ordering Traditional Crystallization Detergent Solutions Lipid Phase Behavior Viscous Mesophase Materials LCP (Lipid Cubic Phase) Syringe Mixer Reconstitution Lipid Bilayer Assay Plate Crystallization Plate
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