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Screening for Melanoma Modifiers anvendelse af et zebrafisk Autokton tumormodel
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Screening for Melanoma Modifiers anvendelse af et zebrafisk Autokton tumormodel

Article DOI: 10.3791/50086-v • 10:23 min • November 13th, 2012
November 13th, 2012 •
1, 2,3, 1
1Program in Molecular Medicine and Department of Cancer Biology, University of Massachusetts Medical School, 2Departments of Surgery and Medicine, Weill Cornell Medical College, 3Departments of Surgery and Medicine, New York Presbyterian Hospital

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En hurtig måde at screene for melanom modifikatorer anvendelse af en zebrafisk autokton tumormodel er præsenteret. Den udnytter den miniCoopR vektor, som tillader ekspression af kandidat melanom gener i melanocytter. En fremgangsmåde til opnåelse af melanom-free overlevelseskurverne, en invasion assay, en protokol til antistoffarvning af skala melanocytter og en melanom transplantation assay er beskrevet.

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Cancer Biology medicin genetik molekylærbiologi melanom zebrafisk, Melanocytter tumor model miniCoopR
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