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Journal / Immunology and Infection / Fluorescencia
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Immunology and Infection

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Fluorescencia

Article DOI: 10.3791/51030-v • 10:38 min • February 24th, 2014
February 24th, 2014 •
1,2, 1, 1, 1, 1, 1, 1,3, 4, 2, 1
1Department of Entomology, Volcani Center, 2Institute of Plant Sciences and Genetics in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, 3Department of Applied Sciences, Institute for Adriatic Crops and Karst Reclamation, 4The Institute of Plant Sciences, Volcani Center

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Se describe aquí un fluorescencia simple en la hibridación in situ (FISH) método para la localización de virus y bacterias en los tejidos de insectos y plantas. Este protocolo se puede ampliar para la visualización de mRNA en todo el montaje y secciones microscópicas.

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Infección FISH localización insectos plantas virus endosymbiont transcripción la fijación la microscopía confocal
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