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Neuroscience

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JoVE Journal Neuroscience
Identificeren van transcriptie Factor Olig2 Genomic bandplaatsen in acuut gezuiverd PDGFRα + cellen door lage-cel chromatine Immunoprecipitation Sequencing analyse
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Identificeren van transcriptie Factor Olig2 Genomic bandplaatsen in acuut gezuiverd PDGFRα + cellen door lage-cel chromatine Immunoprecipitation Sequencing analyse

Article DOI: 10.3791/57547-v • 12:29 min • April 16th, 2018
April 16th, 2018 •
1, 1, 1, 1
1The Vivian L. Smith Department of Neurosurgery, Center for Stem Cell and Regenerative Medicine, University of Texas Health Science Center

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Hier presenteren we een protocol dat is ontworpen voor het analyseren van de genoom-brede binding van de transcriptiefactor Oligodendrocyt 2 (Olig2) in acuut gezuiverde oligodendrocyte voorloper hersencellen (OPCs) door het uitvoeren van lage-cel chromatine immunoprecipitation (ChIP) , bibliotheek voorbereiding, high-throughput sequencing en bioinformatic data-analyse.

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Neurowetenschappen kwestie 134 OPCs Olig2 laag-cel ChIP-seq transcriptionele regulatie immunopanning MACS2
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