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Udtryk for fluorescerende Fusion proteiner i Murine knoglemarv-afledte dendritiske celler og makrofager

Article DOI: 10.3791/58081-v • 09:07 min • October 30th, 2018
October 30th, 2018 •
1,2, 1,2,3, 1,2, 1
1Laboratory of Leukocyte Signalling, Institute of Molecular Genetics of the ASCR, 2Faculty of Science, Charles University, 3Department of Biophysical Chemistry, J. Heyrovsky Institute of Physical Chemistry ASCR

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I denne artikel vil give vi en detaljeret protokol for udtryk for fluorescerende fusion proteiner i murine knoglemarv afledt dendritiske celler og makrofager. Metoden er baseret på transduktion af knoglemarv stamceller med retroviral konstruktioner efterfulgt af differentiering af makrofager og dendritiske celler i vitro.

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Biologi spørgsmål 140 dendritiske celler makrofager myeloide celler differentiering murine knoglemarv celler cytokiner M-CSF GM-CSF virusinfektion normal god landbrugspraksis markeret protein
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