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Journal / Developmental Biology / Generierung induzierter neuronaler Stammzellen…
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Developmental Biology

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JoVE Journal Developmental Biology
Generierung induzierter neuronaler Stammzellen aus peripheren mononukleären Zellen und Differenzierung zu dopaminergen Neuronenvorstufen für Transplantationsstudien
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Generierung induzierter neuronaler Stammzellen aus peripheren mononukleären Zellen und Differenzierung zu dopaminergen Neuronenvorstufen für Transplantationsstudien

Article DOI: 10.3791/59690-v • 12:13 min • July 11th, 2019
July 11th, 2019 •
1,2, 1,2
1Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University, and Key Laboratory of Neurodegeneration, Ministry of Education, 2Center of Neural Injury and Repair, Beijing Institute for Brain Disorders

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Das Protokoll stellt die Reprogrammierung von peripheren mononukleären Blutzellen vor, um neuronale Stammzellen durch Sendai-Virusinfektion zu induzieren, Differenzierung von iNSCs in dopaminerge Neuronen, Transplantation von DA-Vorstufen in die einseitig Parkinson-Mausmodelle und Bewertung der Sicherheit und Wirksamkeit von iNSC-abgeleiteten DA-Vorstufen für die PD-Behandlung.

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Entwicklungsbiologie Ausgabe 149 Neurowissenschaften induzierte neuronale Stammzellen Parkinson-Krankheit Reprogrammierung Differenzierung Transplantation periphere mononukleäre Blutzellen Sendai-Virus 6-OHDA dopaminerge Neuronen
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