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Journal / Immunology and Infection / 使用基于路西法抹去的生长测定，确定化学抑制剂对细胞内弓形体贡迪生长的抑制效率…
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Immunology and Infection

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JoVE Journal Immunology and Infection
使用基于路西法抹去的生长测定，确定化学抑制剂对细胞内弓形体贡迪生长的抑制效率
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Journal (Immunology and Infection)
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使用基于路西法抹去的生长测定，确定化学抑制剂对细胞内弓形体贡迪生长的抑制效率

Article DOI: 10.3791/60985-v • 09:49 min • April 29th, 2020
April 29th, 2020 •
*1,2, *1,2, 1,2, 1,2, 1,2, 1,2
1Department of Biological Sciences, Clemson University, 2Eukaryotic Pathogens Innovation Center, Clemson University
* These authors contributed equally

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本文介绍的是一种协议，用于使用基于荧光酶的生长测定来评估化合物对弓形体淋酸体内生长的抑制作用。该技术用于通过基因删除相应的靶基因来确认抑制特异性。以LHVS对TgCPL蛋白酶的抑制为例。

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免疫学和感染，第158期，弓形虫，CRISPR-Cas9，基因操作，透明酶，生长测定，抑制剂疗效，寄生虫
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