Enriching for Males: Maintaining a Stock of Male C. elegans

Abstract

Source: Singaravelu, G., et al. Isolation and In vitro Activation of Caenorhabditis elegans Sperm. J. Vis. Exp. (2011).

Nematodes can be propagated easily in the lab–in part because hermaphrodites can reproduce by self-fertilization.  However, genetic crosses and other experiments may require a large number of males.  This video describes a method to enrich for male C. elegans.

Protocol

This protocol is an excerpt from Singaravelu et al, Isolation and In vitro Activation of Caenorhabditis elegans Sperm, J. Vis. Exp. (2011). 1. Enrichment of male population Depending on the experimental need, large numbers of males can be obtained by employing one of the following strate…