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Biology
通过飞沫标题细胞封装
Published: October 01, 2007
doi:
10.3791/316
Sangjun Moon
2
,
Pei-Ann Lin
2
,
Hasan Onur Keles
2
,
Seung-Schick Yoo
,
Utkan Demirci
2,4
1
Bio-Acoustic-MEMS Laboratory in Medicine (BAMM), HST-Center for Bioengineering, Brigham and Women’s
,
Harvard Medical School
,
2
Bio-Acoustic-MEMS Laboratory in Medicine (BAMM), HST-Center for Bioengineering
,
Brigham and Women’s Hospital
,
3
Brigham and Women’s Hospital
,
Harvard Medical School
,
4
Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology; Center for Biomedical Engineering, Department of Medicine
,
Brigham and Women’s Hospital
Protocol
NIH3T3细胞的准备: A.弹出细胞 Trypsinize细胞与细胞媒体,然后稀释1:1,从T75烧瓶中转移到一个15毫升的猎鹰管分拆成颗粒细胞,离心,吸出上清液,用DPBS细胞成颗粒细胞再次离心,吸上清在媒体的悬浮细胞确定细胞密度与血球(〜200 × 10 4细胞/ mL的容量瓶中每T75) 离心细胞液,吸出上清液,并悬浮在适量的媒体不同的细胞浓度<…
Disclosures
The authors have nothing to disclose.
Materials
Material Name
Type
Company
Catalogue Number
Comment
Fluorescent Microscope
Nikon
Eclipse TE-2000 U
Solenoid valve cell ejector
Operation frequency: 1 KHz, 30psi, 50nl~0.5ul. 12V Valve Driver: 2.5 Amp drive current
5-Gallon Portable air tank
Coleman
Powermate CT5
Pressurized air: 30 PSI
Pressure regulators
Marsh Bellofram
Pulse Generator
HP8112A
Actuation frequency generation: 50 MHz
XYZ-stage
Newmark Systems
With Stepping motor: 6inch travel xy-stage(NLS4-6-25), 2.5inch travel z-stage(NLS4-2.5-25), 3axis controller(NSC-G3), 0.1um resolution
NIH 3T3
Cell-line
fibroblasts
Trypsin
0.05% solution
NIH 3T3 cell medium
DPBS
Buffer
T75
Tissue culture flasks
Plastic conical tubes
15 ml, for tissue culture
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Tags
Cell Encapsulation
Droplet Encapsulation
Droplet-based Encapsulation
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Cite This Article
Moon, S., Lin, P., Keles, H. O., Yoo, S., Demirci, U. Title Cell Encapsulation by Droplets.
J. Vis. Exp.
(8), e316, doi:10.3791/316 (2007).
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