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Biology
תא כותרת Encapsulation על ידי טיפות
Published: October 01, 2007
doi:
10.3791/316
Sangjun Moon
2
,
Pei-Ann Lin
2
,
Hasan Onur Keles
2
,
Seung-Schick Yoo
,
Utkan Demirci
2,4
1
Bio-Acoustic-MEMS Laboratory in Medicine (BAMM), HST-Center for Bioengineering, Brigham and Women’s
,
Harvard Medical School
,
2
Bio-Acoustic-MEMS Laboratory in Medicine (BAMM), HST-Center for Bioengineering
,
Brigham and Women’s Hospital
,
3
Brigham and Women’s Hospital
,
Harvard Medical School
,
4
Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology; Center for Biomedical Engineering, Department of Medicine
,
Brigham and Women’s Hospital
Protocol
NIH3T3 תאים הכנה: א תאים לניתוק Trypsinize תאים, ואז לדלל 1:01 עם התקשורת התא, ולהעביר מבקבוקון T75 כדי צינור פלקון 15 מ"ל ספין למטה תאים לתוך גלולה ידי centrifuging, לשאוב…
Disclosures
The authors have nothing to disclose.
Materials
Material Name
Type
Company
Catalogue Number
Comment
Fluorescent Microscope
Nikon
Eclipse TE-2000 U
Solenoid valve cell ejector
Operation frequency: 1 KHz, 30psi, 50nl~0.5ul. 12V Valve Driver: 2.5 Amp drive current
5-Gallon Portable air tank
Coleman
Powermate CT5
Pressurized air: 30 PSI
Pressure regulators
Marsh Bellofram
Pulse Generator
HP8112A
Actuation frequency generation: 50 MHz
XYZ-stage
Newmark Systems
With Stepping motor: 6inch travel xy-stage(NLS4-6-25), 2.5inch travel z-stage(NLS4-2.5-25), 3axis controller(NSC-G3), 0.1um resolution
NIH 3T3
Cell-line
fibroblasts
Trypsin
0.05% solution
NIH 3T3 cell medium
DPBS
Buffer
T75
Tissue culture flasks
Plastic conical tubes
15 ml, for tissue culture
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Tags
Cell Encapsulation
Droplet Encapsulation
Droplet-based Encapsulation
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Cite This Article
Moon, S., Lin, P., Keles, H. O., Yoo, S., Demirci, U. Title Cell Encapsulation by Droplets.
J. Vis. Exp.
(8), e316, doi:10.3791/316 (2007).
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