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Immunology and Infection

L'uso di interferone-γ Enzyme-linked Assay Immunospot per caratterizzare Nuovi epitopi T-cellulari di Papillomavirus Umano

Published: March 8, 2012 doi: 10.3791/3657


Name Company Catalog Number Comments
CD8 T cell isolation kit Miltenyi Biotec 130-094-156
Phytohemagglutinin Remel, Thermo Fisher Scientific R-30852801 2mg/vial
Yssel’s medium Gemini Bio Products 400-102 1% pooled human serum
96-well round bottom plate BD Biosciences 08-772-17
Recombinant human IL-2 R&D Systems 202-IL-050 50μg
24-well plate Corning 07-200-84
Phosphate-buffered saline Cellgro MT-21-031-CV
Primary anti-IFN-γ monoclonal antibody (D1K) Mabtech 3420-3-1000
Biotin-conjugated anti-IFN-γ monoclonal antibody (7B-6) Mabtech 3420-6-1000
Multiscreen HA ELISPOT plate EMD Millipore MAHA S45 10
Dimethyl Sulphoxide Sigma-Aldrich D2650 100 ml bottle
Pooled Serum, human Atlanta Biologicals S40510H Heat inactivate
RPMI 1640 Cellgro MT-10-040-CV
Tween-20 Sigma-Aldrich P2287-100ml
Vectastain Elite ABC Kit Vector Laboratories NC9313719
Stable diaminobenzidine Open Biosystems MBI 1241
AID EliSpot Reader Classic Autoimmun Diagnostika GmbH ELR06



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L'uso di interferone-γ Enzyme-linked Assay Immunospot per caratterizzare Nuovi epitopi T-cellulari di Papillomavirus Umano
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Cite this Article

Wang, X., Greenfield, W. W.,More

Wang, X., Greenfield, W. W., Coleman, H. N., James, L. E., Nakagawa, M. Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus. J. Vis. Exp. (61), e3657, doi:10.3791/3657 (2012).

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