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Сотовые слежения Использование Photoconvertible белков в процессе развития данио рерио

Published: September 28, 2012 doi: 10.3791/4350


Name Company Catalog Number Comments
Necessary equipment for raising fish and collecting eggs (see the Zebrafish Book22 for details).
Stereomicroscope Leica Lecia MZ6 Stereomicroscope Equipped with laser operating at 488 nm
Confocal laser scanning microscope Zeiss Zeiss LSM 710 NLO equipped with lasers operating at 488 nm, 561 nm, and 405 nm
Heating block
Pair of Dumont #5 forceps
Cover-glass-bottomed culture dish MatTek Corp Ashland MA USA
Microloader Pipette Tips Eppendorf
Egg water medium
60 μg/ml Instant Ocean Sea Salts in ddH2O
E3 medium
5 mM NaCl
0.17 mM KCl
0.33 mM CaCl2
0.33 mM MgSO4
0.00001% (w/v) Methylene Blue
4 mg/ml Tricaine stock solution
400 mg Tricaine (3-amino benzoic acidethylester) Sigma-Aldrich
97.9 ml ddH2O
2.1 ml 1M Tris (pH9)
Adjust at pH 7, aliquot and store -20 °C
1% LMA (Low melting temperature agarose, Lonza)
1g LMA/100 ml E3 medium 1X
Aliquot and store at 4 °C



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Сотовые слежения Использование Photoconvertible белков в процессе развития данио рерио
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Lombardo, V. A., Sporbert, A.,More

Lombardo, V. A., Sporbert, A., Abdelilah-Seyfried, S. Cell Tracking Using Photoconvertible Proteins During Zebrafish Development. J. Vis. Exp. (67), e4350, doi:10.3791/4350 (2012).

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