Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence

Summary

Here, we describe protocols for the genetic and chemical validation of c-Fos and Dusp1 as a drug target in leukemia using in vitro and in vivo genetic and humanized mouse models. This method can be applied to any target for genetic validation and therapeutic development.

Abstract

The demonstration of tyrosine kinase inhibitors (TKIs) in treating chronic myeloid leukemia (CML) has heralded a new era in cancer therapeutics. However, a small population of cells does not respond to TKI treatment, resulting in minimal residual disease (MRD); even the most potent TKIs fail to eradicate these cells. These MRD cells serve as a reservoir to develop resistance to therapy. Why TKI treatment is ineffective against MRD cells is not known. Growth factor signaling is implicated in supporting the survival of MRD cells during TKI treatment, but a mechanistic understanding is lacking. Recent studies demonstrated that an elevated c-Fos and Dusp1 expression as a result of convergent oncogenic and growth factor signaling in MRD cells mediate TKI resistance. The genetic and chemical inhibition of c-Fos and Dusp1 renders CML exquisitely sensitive to TKIs and cures CML in both genetic and humanized mouse models. We identified these target genes using multiple microarrays from TKI-sensitive and -resistant cells. Here, we provide methods for target validation using in vitro and in vivo mouse models. These methods can easily be applied to any target for genetic validation and therapeutic development.

Introduction

Constitutive tyrosine kinase activity of BCR-ABL1 fusion oncogene causes CML, which provides a rationale to target the kinase activity by small molecule inhibitors. The success of TKIs in treating CML patients revolutionized the concept of targeted therapy^1,2. Subsequently, anti-kinase therapy as precision medicine was developed for several other malignancies, including solid tumors. So far, more than thirty kinase inhibitors have been approved by the United State FDA for treating various malignancies. While TKI treatment is very effective in suppressing the disease, it is not curative. Besides, a small population of cancer cells persists during the treatment: the MRD^3,4,5. Even patients who showed complete remission are left with MRD, which eventually results in relapse if not continuously suppressed. Therefore, the eradication of MRD cells is needed to achieve a durable or curative response. CML represents a valuable paradigm for defining the concept of precision medicine, mechanisms of oncogenesis, rational target-directed therapeutics, disease progression, and drug resistance. However, even today, the mechanism driving TKI-induced cell death in cancer cells is not fully understood, nor why MRD cells (comprised of leukemic stem cells [LSCs]) are intrinsically resistant to TKIs^4,6. Nonetheless, the phenomenon of "oncogene dependence" to mutant kinase oncoprotein is implicated in TKI efficacy where the acute inhibition of targeted oncogene by TKIs causes an oncogenic shock that leads to a massive proapoptotic response or quiescence in cell context-dependent manner^6,7,8,9. However, the mechanistic underpinning of oncogene dependence is lacking. Recent studies have implicated that growth factor signaling abrogates oncogene dependence and consequently confers resistance to TKI therapy^10,11,12. Therefore, to gain insight into the mechanism of oncogene dependence, we performed whole-genome expression profiling from BCR-ABL1 addicted and nonaddicted cells (grown with growth factors), which revealed that c-Fos and Dusp1 are critical mediators of oncogene addiction¹³. The genetic deletion of c-Fos and Dusp1 is synthetic lethal to BCR-ABL1-expressing cells and the mice used in the experiment did not develop leukemia. Moreover, the inhibition of c-Fos and DUSP1 by small molecule inhibitors cured BCR-ABL1-induced CML in mice. The results show that the expression levels of c-Fos and Dusp1 define the apoptotic threshold in cancer cells, such that lower levels confer drug sensitivity while higher levels cause resistance to therapy¹³.

To identify the genes driving the oncogene dependence, we performed several whole-genome expression profiling experiments in the presence of growth factor and a TKI (imatinib) using both mouse- and CML patient-derived cells (K562). These data were analyzed in parallel with CML patient data sets obtained from CD34⁺ hematopoietic stem cells before and after treatment with imatinib. This analysis revealed three genes (a transcription factor [c-Fos], dual specificity phosphatase 1 [Dusp1], and an RNA-binding protein [Zfp36]) which are commonly upregulated in TKI-resistant cells. To validate the significance of these genes in conferring drug resistance, we carried out step-by-step in vitro and in vivo analysis. The expression levels of these genes were confirmed by real-time qPCR (RT-qPCR) and western blotting in drug-resistant cells. Further, cDNA overexpression and knockdown by shRNA hairpins of c-Fos, Dusp1, and Zfp36 revealed that elevated c-Fos and Dusp1 expressions are sufficient and necessary to confer TKI resistance. Therefore, we performed an in vivo validation using mouse models with c-Fos and Dusp1 only. For the genetic validation of c-Fos and Dusp1, we created ROSACreERT-inducible c-Fos^fl/fl mice (conditional knockout)¹⁴ and crossed them with Dusp1^-/- (straight knockout)¹⁵ to make ROSACreERT2-c-Fos^fl/flDusp1^-/- double-transgenic mice. Bone marrow-derived c-Kit⁺ cells (from c-Fos^fl/fl-, Dusp1^-/--, and c-Fos^fl/flDusp1^-/-) expressing BCR-ABL1 were analyzed in vitro in a colony-forming unit (CFU) assay, and in vivo by bone marrow transplantation in lethally irradiated mice, to test the requirement of c-Fos and Dusp1 alone or together in leukemia development. Likewise, the chemical inhibitions of c-Fos by DFC (difluorinated curcumin)¹⁶ and Dusp1 by BCI (benzylidene-3-(cyclohexylamino)-2,3-dihydro-1H-inden-1-one)¹⁷ were tested in vitro and in vivo using BCR-ABL1-expressing, bone marrow-derived c-Kit⁺ cells from the wild-type (WT) mouse. To confirm the requirement of c-Fos and Dusp1 in leukemic stem cells, we utilized a CML mouse model where BCR-ABL1 was specifically induced in its stem cells by doxycycline (Tet-transactivator expresses under murine stem cell leukemia (SCL) gene 3' enhancer regulation)^18,19. We used bone marrow Lin^-Sca⁺c-Kit⁺ (LSK) cells from these mice in an in vivo transplant assay. Furthermore, we established phopsho-p38 levels and the expression of IL-6 as pharmaco-dynamic biomarkers for Dusp1 and c-Fos inhibition, respectively, in vivo. Finally, to extend the study for human relevance, patient-derived CD34⁺ cells (equivalent to the c-Kit⁺ cells from mice) were subjected to long-term in vitro culture-initiating cell assays (LTCIC) and an in vivo humanized mouse model of CML^20,21. The immunodeficient mice were transplanted with CML CD34+ cells, followed by drug treatment and analysis of human leukemic cell survival.

In this project, we develop methods for target identification and validations using both genetic and chemical tools, using different preclinical models. These methods can be successfully applied to validate other targets developing chemical modalities for therapeutic development.

Protocol

All animal experiments were carried out according to the guidelines of the Institutional Animal Care and Use Committee (IACUC) at Cincinnati Children's Hospital Medical Center (CCHMC). Human specimens (Normal BM and that from CML (p210-BCR-ABL+) leukemia) were obtained through Institutional Review Board-approved protocols (Institutional Review Board: Federalwide Assurance #00002988 Cincinnati Children's Hospital Medical Center) and donor-informed consent from CCHMC and the University of Cincinnati.

1. Real-time qPCR Analysis

1. Isolate total RNA from BaF3 cells growing in RPMI supplemented with 10% FBS and 10% WEHI spent culture medium as a source of interleukin-3 (IL-3).
2. Harvest the cells at logarithmic phase (99% alive) from treated (IL-3 and imatinib) as well as from untreated samples, and centrifuge them at 435 x g for 3 min. The health and the growth stages of the cells are critical as their gene expression profiles can change dramatically.
3. Isolate RNA by purifying total RNA²². Quantify the total RNA; then, subject it to DNase treatment²³. Convert equal amounts of DNA-free RNA (2 µg) to cDNA with reverse transcriptase²⁴.
4. Perform qPCRs with the gene-specific primers (Table 1). Carry out PCRs in 20 µL reactions in 0.2 mL, thin-walled PCR tubes with optical caps, using a 1x polymerase mix (see Table of Materials), 0.5 µM of each primer, 1 µL of DNA, and water. Place the reaction in the thermocycler with the following cycle: Step 1, 95 °C for 10 min; Step 2, 95 °C for 15 s; Step 3 at 60 °C for 1 min; Step 4, repeat steps 2–3 40 times. Perform all PCRs in triplicates and normalize the real-time data to the β-actin expression before plotting.

2. Western Blotting

NOTE: Whole-cell extracts were prepared by adding 250 µL of 1x lysis buffer as described in Kesarwani et al.¹³ supplemented with a protease inhibitor cocktail, and phosphatase inhibitor cocktail 2.

1. Harvest five million BaF3 cells at logarithmic phase (99% alive) from treated (IL-3 and imatinib) as well as from untreated BaF3 cells by centrifugation at 435 x g for 3 min at 4 °C. Lyse the cells in the lysis buffer (250 µL) by pipetting up and down 1x, followed by a 5 min incubation at 80 °C. Sonicate the lysate to break down all the DNA with 5–6 pulses of 5 s each in a cold room at 4 °C.
2. Transfer the lysate to a 1.5 mL tube and centrifuge it at 16,000 x g for 5 min to remove any insoluble material. The extract can be stored at -80 °C until use. Heat samples to 80 °C for 5 min in a heat block before loading. Load 10 µL of the sample using a gel-loading tip on 10% SDS-PAGE gel.
3. Resolve the proteins at 150 V until the reference dye reaches the bottom of the gel. Transfer the protein to nitrocellulose membranes by an electrophoretic transfer at 1 amp for 1 h. After the transfer, block the membranes in 1x Tris-buffered saline + 0.1% Tween 20 (TBST) with 5% non-fat milk for 1 h and probe overnight at 4 °C with the appropriate antibodies at a 1:1,000 dilution.
4. Wash the membranes 3x to 5x with TBST for 10 min each; then, probe with an HRP-conjugated appropriate secondary antibody (1:5,000 dilution) at room temperature for 1 h. The amount of TBST used depends on the container size. Make sure to submerge the membrane in TBST completely. Wash the secondary antibody 3x for 5 min each with TBST.
5. Develop the blot using chemiluminescent substrate reagent. Mix equal volumes of the substrate and the buffer from the two bottles, right before use. Add the substrate to the top of the membrane to cover the whole membrane with a 1 mL pipette and incubate for 1 min. The blots should be imaged within 1–10 min of adding the substrate.
6. Using blunt forceps, carefully place the membrane on the platform of the imaging system (see Table of Materials), avoiding much of the liquid. Select a live view and choose blots and chemiluminiscence from the menu. Using manual acquisition, acquire multiple exposures at different time points. These files can be visualized and quantified using imaging software.

3. Generation of Knockout Mice

1. Cross c-Fos^fl/fl mice with ROSACreERT2 mice to generate ROSACreERT2c-Fos^fl/fl mice¹³. To create the double-knockout (KO) mice, breed ROSACreERT2c-Fos^fl/fl mice with Dusp1^-/- mice to generate ROSACreERT2c-Fos^fl/fl Dusp1^-/- mice¹³. Genotype the mice by tail clips followed by PCR, as described below.
2. Genotyping:
   1. To prepare the DNA, clip a tiny portion of the tail with scissors and put it into a 1.5 mL tube. Cauterize the tail with heated scissors. Add 200 µL of 50 mM NaOH to the clipped tail in the tube and incubate at 95 °C in a heat block for 1 h.
   2. Vortex the tube well and, then, neutralize by adding 50 µL of 1 M Tris HCl pH 6.8 and vortex again. Centrifuge the tube at maximum speed for 1 min.
   3. Carry out PCRs in 20 µL reactions in 0.2 mL, thin-walled PCR tubes using 1x Taq polymerase master mix containing dye, 0.5 µM of each primer, 1 µL of DNA, and water. Place the tubes in the thermocycler and run the appropriate cycles as shown in Table 2.
   4. Run the PCR product on 2% agarose gel and image using a gel documentation system.

4. Isolation of c-Kit⁺ Cells from Bone Marrow

1. Sacrifice the mice according to institutional guidelines.
   NOTE: Here, the mice were exposed to carbon dioxide (CO₂) at an according to the AVMA recommended flow rate until death was determined by the cessation of respiration and heartbeat, followed by cervical dislocation.
2. Harvest the legbones from the mouse — two femurs, two tibias, two iliacs. Clean off all tissues from the bones by scraping away with a scalpel. Put the leg bones in cold 1x PBS on ice — 5 mL in a 15 mL tube. Pour the contents of the tube into a mortar and crush it with a pestle.
3. Filter the cell suspension through a 70 µm cell strainer into a 50 mL screw cap tube with a 10 mL pipette attached, using a pipette. Wash the mortar and pestle multiple times with cold 1x PBS, collecting and adding cell suspension to the 50 mL tube. Spin down bone marrow at 1,200 x g at 4 °C for 5 min. Remove the supernatant via a vacuum with a glass pipette attached. Suspend the cell pellet in 5 mL of 1x PBS with a pipette.
4. With a pipette, slowly add suspended bone marrow to the top of 5 mL of room temperature (the temperature is critical) polysucrose, taking great care not to disturb the interface. Spin at 400 x g at room temperature for 20 min with the brake turned off.
5. Collect the cloudy total mono-nuclear cell (TMNC) interface with a pipette and put it into a new 15 mL tube. Bring the volume up to 10 mL with 1x PBS for the wash, taking 20 µL for counting, and spin at 1,200 x g at 4 °C for 5 min. Discard the supernatant and save the pellet on ice for step 4.6.1.
6. Perform c-Kit⁺ cell isolation.
   1. Follow a microbead kit protocol for the c-Kit⁺ cell isolation. Resuspend the cell pellet in 80 µL of 1x PBS + EDTA + BSA to 10⁷ cells. To the cell suspension, add 20 µL of CD117 MicroBeads/10⁷ total cells. Mix well by vortexing and incubate for 10 min at 4 °C. Bring the volume up to 10 mL by adding 1x PBS + EDTA + BSA and spin at 1,200 x g at 4 °C for 5 min.
   2. Remove the supernatant via a vacuum and suspend the cell pellet in 500 µL/10⁸ total cells in 1x PBS + EDTA + BSA. Using the magnet stand with the magnetic column attached, add 3 mL of 1x PBS + EDTA + BSA and allow it to flow through via gravity into a 15 mL collection tube.
   3. Once the first buffer addition has finished passing through the column, add the cell suspension to the column, allowing it to flow through via gravity into the 15 mL collection tube. This flowthrough is the undesired cell fraction.
   4. Wash the column 3 times with 3 mL 1x PBS + EDTA + BSA each time, allowing it to flow through via gravity into the collection tube. Remove the column from the magnet and place onto the top of a 15 mL tube.
   5. Add 5 mL of 1x PBS + EDTA + BSA and immediately flush it with the plunger provided with the column. Remove the plunger and repeat the flush with an additional 5 mL of 1x PBS + EDTA + BSA. Count the cells in the total volume using standard methods.
   6. Spin down the cells at 1,200 x g at 4 °C for 5 min. Remove the supernatant and resuspend the pellet in complete IMDM (IMDM + 10% FBS + IL-6 [10 ng/mL] + mSCF [50 ng/mL] + FLT3 ligand [20 ng/mL] + IL-3 [10 ng/mL]) for culture. Culture these cells overnight in 2 x 10⁶ cells/1 mL of cell IMDM complete media by placing them in an incubator at 37 °C and 5% CO₂.

5. Transduction

1. Retroviral plasmid transfection
   1. Freshly thaw HEK293 cells the afternoon before in a water bath set to 37 °C. Spin the cryotube containing the HEK cells at 435 x g at room temperature for 3 min. Remove the supernatant via a vacuum and suspend the cell pellet in 1 mL of DMEM supplemented with 10% FBS, penicillin, streptomycin, and glutamine.
   2. Count the cells and add the appropriate volume to a 10 cm treated dish (~4 x 10⁶ HEK293T cells). Add 14 mL of the DMEM 10 media to the dish and mix it well by sliding up and down for an equal distribution. Place the dish in a 37 °C, 5% CO₂ incubator overnight.
   3. On the next morning, check the confluence of the cells under an inverted light microscope (50% or more works well).
   4. Combine the DNA (desired retroviral plasmid), PclEco (packaging plasmid), 2 M CaCl₂, and H₂0 in a 1.5 mL tube (= 500 µL) using a micropipette in the following amounts: 7.5 µg of DNA (BCR-ABL1-YFP), 7.5 µg of pCLeco, 62 µL of 2 M CaCl₂, and water to a final volume of 500 µL. Add 500 µL of 2x HBS to another 1.5 mL tube.
   5. Add the DNA mix dropwise to the 1.5 mL tube containing 500 µL of 2x HBS (280 mM NaCl, 100 mM HEPES, and 1.5 mM Na₂HPO₄) while mixing. Achieve this by setting a vortex to the lowest speed and hold the HBS tube on it for constant mixing while adding the transfection mix.
   6. Allow the transfection mix to incubate for 20–30 min at room temperature. During the incubation, add 25 nM chloroquine to the HEK cells and place them back in the incubator. After the incubation, add the transfection mix dropwise to the HEK cells and, then, gently swirl the media to mix the transfection mix throughout the plate.
   7. Incubate the cells with the mix for ~8 h in a 37 °C, 5% CO₂ incubator. Change the media on these cells by very slowly adding 14 mL of fresh DMEM 10 media. Pipetting slowly against the wall of the dish helps to prevent any stripping of the HEK cells. Incubate the cells overnight in the 37 °C, 5% CO₂ incubator.
   8. Collect the viral supernatant with a 10 mL syringe, draw the supernatant into the syringe, and attach the 0.45 µm syringe filter. Plunge the viral supernatant into a new collection tube. Take the first collection of viral supernatant ~16 h later.
2. Fibronectin viral concentration and transduction
   1. Add 2 mL of a 0.1 mg recombinant human fibronectin fragment in 1x PBS to untreated 6-well culture plates. Prepare as much fibronectin as needed, which depends on the number of cells and genotypes. One well can sufficiently transduce 10 million c-Kit⁺ cells. Incubate the plate overnight at 4 °C.
   2. The next day, remove the fibronectin from the wells with a pipette, pipette 2 mL of sterile 2% BSA in 1x PBS to block the plate, and incubate for 30 min at room temperature. Remove the BSA via a vacuum and wash thoroughly by pipetting 2 mL of 1x PBS then remove it via a vacuum.
   3. Immediately add freshly collected and filtered (at 0.45 µm) viral supernatant up to 5 mL. Centrifuge at 435 x g at 32 °C for 2 h. Remove the supernatant via a vacuum and repeat this step with additional freshly collected viral supernatant and spin again. Remove the supernatant via a vacuum and wash the wells by adding 2 mL of 1x PBS; then, remove it via a vacuum.
   4. Add the c-Kit⁺ mouse cells that were cultured overnight (step 4.6.6) to the viral coated wells by mixing the cell suspensions well and pipetting them into the now viral concentrated fibronectin plate. Spin at 1,200 x g at 25 °C for 90 min. Put the cells in a 37 °C, 5% CO₂ incubator.
   5. 48 h posttransduction, pellet the cells by centrifugation at 1,200 x g at 4 °C for 5 min and resuspend them in FACS buffer #1 (1x PBS + 0.5% BSA) to 6 x 10⁶/mL. Determine the percentage of BCR-ABL1 positive cells by measuring the percentage of YFP positive using flow cytometry.
   6. Acquire events with the standard procedure. First, gate the live cells based on forward and side scatter. Then, gate the YFP positive live cells excluding the YFP-negativecells determined by negative control (Figure 4).

6. Colony-forming UnitAssays

1. Thaw the methylcellulose with cytokines for mouse at room temperature for 1–2 h. Aliquot 3 mL of methylcellulose in a FACS tube using a 5 mL syringe without a needle. Sort the YFP-positive cells on a cell sorter.
2. Spin the cells down and suspend the pellet in IMDM complete media. Count the cells to be plated and dilute them to obtain 5,000 YFP-positive cells in 100 µL of IMDM complete media.
3. Add 100 µL of the cell suspension containing 5,000 YFP-positive cells and the appropriate inhibitors to 3 mL of methylcellulose (see Table of Materials). Vortex on high to mix it for 10–30 s.
4. After most of the air bubbles have escaped, use a 1 mL syringe to plate 1 mL of media in three replicate 3 cm plates by ejecting the media on one side and slowly tilting the plate in a circular motion to spread evenly.
5. The final concentration of the inhibitors to use are imatinib at 3 µM, DFC at 0.2 µM, and BCI at 0.5 µM, alone or in combinations. Wherever appropriate, delete c-Fos by plating the cells with 4-hydroxytamoxifen (1 µg/mL) added to the methylcellulose.
6. After plating, put the plates in a large Petri dish with one open dish containing 2 mL of sterile water in the middle. Cover the large Petri dish and carefully incubate at the 37 °C, 5% CO₂ incubator. Record the colony numbers after one week of plating by counting the total number of colonies under a microscope.
7. Analyze a few colonies from appropriate plates for c-Fos deletion by PCR. Collect the colonies by sucking them with a P1000 tip. Dislodge the cells in 500 µL of 1x PBS by washing the tip in the PBS multiple times. Spin the cell suspensions at 6,000 x g for 1 min and extract the DNA from the cell pellet using a genomic DNA isolation kit.
8. Use the genomic DNA for PCR using the primers mFos-FP3 and mFos-RP5, as described in Table 2. Analyze the PCR products on a 2% agarose gel and image using a gel documentation system.
9. For a graphic presentation of the colonies, stain the colonies, using Iodonitrotetrazolium chloride. Dissolve 10 mg of the Iodonitrotetrazolium chloride in 10 mL of water to obtain a 1 mg/mL solution. Filter sterilize the solution using a Luer lock with a 0.2 µm filter attached to a 10 mL syringe under sterile conditions in a tissue culture hood.
10. In the tissue culture hood, add 100 µL of staining solution dropwise around the CFU plate; be careful not to slide or disturb colonies. Incubate the plates overnight in a 37 °C, 5% CO₂ cell culture incubator. The colonies will turn dark reddish brown. Using a white background in the sterile tissue culture hood, take photos of the stained CFU plate.

7. Transplantation and Mortality Assay

1. Lethally irradiate the mice with split radiation using a 137 Cs-based Gamma-ray at a dosage of 7.0 Gy followed by 4.75 Gy (0.5 Gy/min), 3 h apart before the transplant.
2. Transplant 4 x 10⁴ unsorted YFP-positive (calculated based on the percentage of YFP) cells with 3 x 10⁵ normal bone marrow cells as a carrier into each mouse through tail vein injection.
3. After one week of transplantation, determine the engraftment by analyzing the YFP-positive cells from the peripheral blood using FACS.
4. Perform a tail vein bleed and FACS analysis.
   1. Warm the cage of mice under a heat lamp with caution not to overheat or burn them.
   2. Restrain a mouse in a mouse restrainer and nick the tail vein with a sterile blade. Gently milk the tail from anterior to posterior, allowing a blood drop to accumulate. Collect the blood with a heparinized capillary tube until it is filled (collect around 75 - 100 µL). Plunge the blood from the filled capillary tube into a blood collection tube containing EDTA and mix well.
   3. Lyse the RBCs by adding 30–40 µL of blood to 3 mL of 1x RBC lysis buffer (150 mM ammonium chloride, 10 mM potassium bicarbonate, and 0.13 mM EDTA). Mix well, inverting the tube multiple times. Incubate at room temperature for 10–15 min. Spin at 1,200 x g at 4 °C for 5 min. Remove the supernatant via a vacuum and suspend the pellet in 2 mL of 1x PBS for a wash.
   4. Spin at 1,200 x g at 4 °C for 5 min. Remove the supernatant and suspend it in FACS buffer #1. Carry out FACS analysis as described above in step 5.2.5–5.2.66. Short-term store the remaining blood in the collection tube, which can be used for various other purposes, at 4 °C.
5. Transplanted mice that showed 10–40% YFP-positive cells in the peripheral blood were used for the experiment. Mice that had less than 2% of YFP-positive cells were excluded from the study.
6. Prepare tamoxifen at 20 mg/mL in corn oil at 60 °C with intermittent vortexing until completely dissolved, and store it at 4 °C in the dark for the duration of the treatment. After one week of transplantation, delete the c-Fos^fl/fl allele by injecting tamoxifen (100 mg/kg in corn oil) intraperitoneally (i.p.) into the mice, every day for three consecutive days. It's important that the mice are weighed to determine how much tamoxifen to inject. After the tamoxifen treatment (where appropriate), group the mice for drug treatments (n = 5/group).
7. Monitor the mice for leukemia progression and survival and determine the leukemic burden (YFP-positive cells by FACS) weekly up to eight weeks in surviving mice.
8. Analyze the blood for c-Fos deletion wherever appropriate, as described above in section 6. Record the number of surviving mice every day and plot the survival curve at the end of the experiment using a graphing software.

8. Transgenic Mice Model of BCR-ABL1 Leukemia

1. LSK isolation
   1. Maintain the Scl-tTA transgenic mice on doxycycline chow to prevent an expression of BCR-ABL1. Four weeks before the transplant, take the mice off the doxycycline chow for BCR-ABL1 expression.
   2. Isolate TMNCs from Scl-tTA transgenic mice as described above in section 4. Suspend the TMNCs in FACS buffer #2 (1x PBS + 0.5% BSA + 2 mM EDTA) to obtain 10⁶ cells/mL.
   3. Deplete the TMNCs for lineage-positive cells by adding 10 µL of lineage cell detection cocktail biotin (biotin-conjugated monoclonal antibodies against CD5 [rat IgG2a], CD11b [rat IgG2a], CD45R [B220] [rat IgG2a], Anti-7-4 [rat IgG2a], Anti-Gr-1 [Ly-6G/C, rat IgG2a], and Anti-Ter-119 [rat IgG2b]) per 1 x 10⁶ cells. Incubate the cells at 4 °C for 10 min in the dark, followed by a wash with 5 mL of FACS buffer #2, and spin at 1,200 x g at 4 °C for 5 min. Aspirate the supernatant completely and suspend the cells in 400 µL of FACS buffer #2.
   4. Add 5 µL of anti-biotin antibody-FITC conjugate, 1.2 µL of Ly-6A/E (Sca1)PECy7, and 2.4 µL of CD117 (c-Kit) APC antibodies for up to 10⁸ million cells. Incubate in the dark at room temperature for 10 min. Wash by bringing the volume up to 5 mL with FACS buffer #2; then, centrifuge at 1,200 x g at 4 °C for 5 min. Remove the supernatant and suspend the cell pellet in 500 µL of FACS buffer #2.
   5. Filter the cell suspension into a blue filter-cap FACS tube.
   6. Gate live cells using forward and side scatter. Then, select the lin^- cells that showed MFI (mean fluorescence intensity of less than 10²) to get LSK cells c-Kit⁺ and Sca1⁺ on a biexponential plot from the Lin^- parent, and sort them (Figure 7C).
   7. Collect the sorted cells and centrifuge them at 1,200 x g at 4 °C for 5 min.
2. Transplantation
   1. Lethally irradiate the BoyJ mice with a split radiation dosage of 7.0 Gy followed by 4.75 Gy after 3 h, before the transplant.
   2. Inject 3 x 10³ to 5 x 10³ BCR-ABL1-LSK cells with 0.3 x 10⁶ helper bone marrow cells from WT BoyJ mice via tail vein (i.v.) into the irradiated BoyJ mice.
   3. Four weeks posttransplantation, analyze the recipient mice for CD45.1 and CD45.2. Obtain the TMNC from the peripheral blood by tail vein bleeding as described in step 7.4. Resuspend the cells in 100 µL of FACS buffer. Incubate the cells with 1 µL each of the CD45.1-FITC and CD45.2-PE antibody at room temperature for 1 h.
   4. Wash the cells by bringing up the volume to 3 mL with FACS buffer and spin at 1,200 x g at 4 °C for 5 min. Remove the supernatant and resuspend it in 200 µL of 1x PBS.
   5. Analyze the percentage on CD45.1 and CD45.2 by first gating the live cells based on forward and side scatter, followed by gating FITC- and PE-positive cells based on the unstained sample.
   6. Group the mice into four groups (n = 6 per group). Start the treatment with imatinib (75 mg/kg 2x daily) alone and in combination with DFC + BCI (both drugs were given at a dose of 10 mg/kg 2x daily).
   7. Likewise, treat other groups with combinations of imatinib (75 mg/kg) + curcumin (150 mg/kg) + BCI (10 mg/kg) and imatinib (75 mg/kg ) + NDGA (100 mg/kg) + BCI (10 mg/kg) for three months, 2x a day, by injected i.p.
   8. Analyze the mice 1x a month for six months for leukemic chimerism by determining the percentage of CD45.2 positive cells in the peripheral blood by tail vein bleeding as described in step 7.4.

9. In Vivo Evaluation of BCI and DFC Activity

1. Phospho-p38 analysis
   1. Take three leukemic mice (8–12 weeks old) that were transplanted with BCR-ABL1-expressing c-Kit⁺ cells, as described in section 7. Inject the mice with BCI (10 mg/kg) by injecting i.p. four weeks posttransplant.
   2. Measure the levels of phospho-p38 peripheral blood TMNC using the phosphorylation flow cytometry kit according to the supplier's instructions. Collect the blood from each mouse before and 6 h after the drug injection. Isolate the TMNCs by RBC depletion and fix them as follows.
   3. Add 4 mL of RBC lysis solution per 100 µL of peripheral blood. Mix and incubate on ice for 5 min to lyse the RBCs. Spin down the cells at 1,200 x g for 5 min at 4 °C and remove the supernatant. Repeat the lysis 1x more.
   4. After the second lysis step, wash the cell pellets with 1 mL of 2% BSA in PBS. Fix the cells by adding 100 µL of fixation and permeabilization solutions and incubate for 20 min at 4 °C in the dark. Pellet the cell by centrifugation at 1,200 x g for 5 min at 4 °C.
   5. Wash the pellets with 1 mL of 1x permeabilization wash. Block the cells by adding 300 µL of 2% BSA in permeabilization wash buffer at room temperature for 20 min. Divide the cells into three equal aliquots of 100 µL each (~1 x10⁶).
   6. To each aliquot of fixed cells add 1 µL of total p-38 antibody, 1 µL of phospho-p38 antibody, or 1 µL of isotype IgG control, respectively, and incubate overnight at 4 °C.
   7. Wash the cells 1x with 5 mL of 2% BSA in PBS and incubate with secondary antibody (1 µL of Alexa Fluor-488 conjugated) for 1 h at room temperature. Wash the cells 1x again and suspend them in 200 µL of 1x PBS.
   8. Acquire the data by FACS and analyze them by flow cytometry analysis software.
   9. Deduct the MFI of the IgG control from the MFI of the experimental samples. The MFI values of phospho-p38 are then normalized to that of total p-38 to determine the phospho-p38 levels after the BCI injection.
2. Quantitative gene expression analysis of c-Fos target genes
   1. Take three leukemic mice (8–12 weeks old), that were transplanted with BCR-ABL1-expressing c-Kit⁺ cells as described in section 7. Collect the peripheral blood from each mouse and then inject the mice with 10 mg/kg each of DFC + BCI.
   2. Collect the peripheral blood 6 h after the drug injection. Isolate TMNCs by RBC depletion, as described above. Pellet the TMNCs and suspend them in a phenol-based lysis buffer (see Table of Materials).
   3. Carry out RT-qPCR analysis (as described in section 1) for Bcl2l11, Lif, and IL-6 using gene-specific primers (shown in Table 1).

10. Long-term Culture-Initiating Cell Assay

NOTE: An LTCIC assay was performed as described previously²⁵.

1. Grow a mouse fibroblast cell line MS-5 in MEMα to confluency in a T175 tissue culture flask. Trypsinize the cells as described above for HEK293T. Suspend the cells in MEMα at 2 x 10⁶ cells/mL. Take 10 x 10⁶ cells in a 50 mL conical tube and irradiate at 80 Gy. Dilute the cells to 0.5 x 10⁶ cells/mL in MEMα supplemented with 10% FBS (M10) media. Plate 2 mL per well in a 12-well plate and incubate it in a 37 °C, 5% CO₂ incubator overnight.
2. On the next day, prepare the hydrocortisone sodium hemisuccinate by dissolving 2.5 mg in 5 mL of MEMα (1 M solution). Filter-sterilize the hydrocortisone solution using a 0.2 µm syringe filter in biosafety hood. Dilute the hydrocortisone by serial dilution in MEM to obtain a 100 µM solution. Add 250 µL of this to 25 mL of human long-term culture medium (HLTM) (see Table of Materials) just prior to use, to achieve a final concentration of 1 µM.
3. Spin down the CML-CD34⁺ and UCP3 TMNCs and suspend them in the HLTM by brief vortexing, and determine the counts by a hemocytometer.
4. Vacuum off the M10 media from the 12-well plate seeded with stroma cells and replace it with the 1 mL of patient cell (CML-CD34⁺ [10,000] and UCP3 TMNCs [1 x 10⁶]) suspension in HLTM. Use three wells per drug combination per cell type.
5. Dilute the drugs stocks to 2x of the required concentration in HLTM. Add 1 mL of the media with drugs to the above-mentioned cells to obtain a 1x drug concentration. Replace one-half of the media with freshly prepared HLTM every week for five weeks.
6. At the end of the six-week LTC-IC assay period, collect nonadherent cells into a tube from the cultures. Trypsinize adherent cells and combine them with the corresponding nonadherent cells.
7. Wash the cells and assay for CFUs in methylcellulose medium containing 30% fetal calf serum, erythropoietin (3 units/mL), SF (50 ng/mL), and IL-3(20 ng/mL), IL-6 (20 ng/mL), G-CSF (20 ng/mL), and granulocyte/macrophage colony-stimulating factor (GM-CSF) (20 ng/mL). Determine the counts by a hemocytometer.
8. Plate 5 x 10⁴ cells in three replicates. Freeze down the remaining cells in 20% FBS and 10% DMSO. Arrange the plates in a large Petri dish with one open dish containing water in the middle. Cover the large Petri dish carefully and incubate it in a 37 °C, 5% CO₂ incubator for two weeks.
9. Score the colonies two weeks later. In some cases, only part of the harvest is assayed for CFUs). Calculate the total number of LTCIC present in the initial cell suspension by extrapolating based on the CFUs obtained from part of the cell harvest. The average CFU output per LTC-IC is 8.
   NOTE: For example, if initially 1 x 10⁶ cells were plated in a well, after five weeks, 0.5 x 10⁶ cells were harvested, and for CFU, 5 x 10⁴ cells were plated and 50 CFUs were obtained. This equals 500 CFUs/1 million plated cells. Divide this number by 8 to get the LTCIC, which is 62.5.

11. Humanized Mouse Model Using CML CD34⁺ Cells

1. Sublethally irradiate the 8 week-old NOD scid gamma mice, expressing human IL3, GM-CSF and SCF (NSGS), with a single dose of 7.0 Gy (700 rads) with 50 rads/min.
2. Inject 3 x 10⁶ CD34⁺ selected cells from BCR-ABL1-positive CML chronic phase patients into the irradiated mice by intravenous injection.
3. After two weeks of transplantation, determine leukemic engraftment in bone marrow by FACS using mouse- and human-specific antibodies against CD45.
4. Perform a FACS analysis.
   1. Take the bone marrow aspirate from the femurs of the transplanted mice. Lyse RBCs using RBC lysis buffer as described above and collect the TMNCs by centrifugation. Wash the pellet 1x with cold 1x PBS.
   2. Block the cells with human FcR block and mouse FcR block followed by staining with anti-human CD45 FITC and anti-mouse CD45 APC^Cy7 overnight at 4 °C. Perform the FACS analysis on LSRII and analyze the data using flow cytometry analysis software.
5. Group the mice into four different cohorts (n = 6/group) for the treatment with vehicle, imatinib (75 mg/kg), DFC + BCI (both at 10 mg/kg), or imatinib (75 mg/kg) + DFC + BCI (both at 10 mg/kg). Dilute the stock drugs in 1x PBS (vehicle) and administer them by injecting them i.p. 2x daily.
6. Treat the mice for six weeks and determine the leukemic burden every two weeks, until week eight after the transplantation as described above in 11.4.

Results

Oncogene addiction has been implicated in the therapeutic efficacy of TKIs. However, the mechanisms driving the oncogene dependence are not understood. We performed multiple unbiased gene expression analyses to identify the genetic component involved in orchestrating the addiction. These analyses revealed the upregulation of three genes, c-Fos, Dusp1, and Zfp36, in cancer cells that are not dependent on oncogenic signaling for survival and, thus, are insensitive to TKI treatment. The down...

Discussion

For the bulk of cancer cells, the therapeutic response to TKI is mediated by a blockade of tyrosine-kinase-oncoprotein signals to which the tumor is addicted. However, relatively little is known about how a minority of cancer cells contributing to MRD escape the oncogene dependence and therapy⁴. Recent studies revealed that growth factor signaling mediates drug resistance in both leukemia and solid organ tumors. This suggests that various molecular mechanisms might underlie intrinsic resistance

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Biological Materials
RPMI	Cellgro (corning)	15-040-CV
DMEM	Cellgro (corning)	15-013-CV
IMDM	Cellgro (corning)	15-016-CVR
RetroNectin Recombinant Human Fibronectin Fragment	Takara	T100B
MethoCult GF M3434 (Methylcellulose for Mouse CFU)	Stem Cell	3434
MethoCult H4434 Classic (Methylcellulose for Human CFU)	Stem Cell	4434
4-Hydroxytamoxifen	Sigma	H6278
Recombinant Murine SCF	Prospec	CYT-275
Recombinant Murine Flt3-Ligand	Prospec	CYT-340
Recombinant Murine IL-6	Prospec	CYT-350
Recombinant Murine IL-7	Peprotech	217-17
DFC	LKT Laboratories Inc.	D3420
BCI	Chemzon Scientific	NZ-06-195
Imatinib	LC Laboratory	I-5508
Curcumin	Sigma	458-37-7
NDGA	Sigma	500-38-9
Penn/Strep	Cellgro (corning)	30-002-CI
FBS	Atlanta biological	S11150
Trypsin EDTA 1x	Cellgro (corning)	25-052-CI
1x PBS	Cellgro (corning)	21-040-CV
L-Glutamine	Cellgro (corning)	25-005-CL	5 mg/mL stock in water
Puromycin	Gibco (life technologies)	A11138-03
HEPES	Sigma	H7006
Na2HPO4.7H2O	Sigma	S9390
Protamine sulfate	Sigma	P3369	5 mg/mL stock in water
Trypan Blue solution (0.4%)	Sigma	T8154
DMSO	Cellgro (corning)	25-950-CQC
WST-1	Roche	11644807001
0.45 μM acro disc filter	PALL	2016-10
70 μm nylon cell stariner	Becton Dickinson	352350
FICOL (Histopaque 1083) (polysucrose)	Simga	1083
PBS	Corning	21040CV
LS Columns	Miltenyi	130-042-401
Protease Inhibitor Cocktail	Roche	CO-RO
Phosphatase Inhibitor Cocktail 2	Sigma	P5762
Nitrocullulose Membrane	Bio-Rad	1620115
SuperSignal West Dura Extended Duration Substrate ( chemiluminiscence substrate)	Thermo Scientific	34075
CD5	eBioscience	13-0051-82
CD11b	eBioscience	13-0112-75
CD45R (B220)	BD biosciences	553092
CD45.1-FITC	eBioscience	11-0453-85
CD45.2-PE	eBioscience	12-0454-83
hCD45-FITC	BD Biosciences	555482
Anti-Biotin-FITC	Miltenyi	130-090-857
Anti-7-4	eBioscience	MA5-16539
Anti-Gr-1 (Ly-6G/c)	eBioscience	13-5931-82
Anti-Ter-119	eBioscience	13-5921-75
Ly-6 A/E (Sca1) PE Cy7	BD	558612
CD117 APC	BD	553356
BD Pharm Lyse	BD	555899
BD Cytofix/Cytoperm (Fixing and permeabilization solution)	BD	554714
BD Perm/Wash  (permeabilization and wash solution for phospho flow)	BD	554723
phospho p38	Cell Signaling Technologies	4511S
total p38	Cell Signaling Technologies	9212
Mouse IgG control	BD	554121
Alexa Flour 488 conjugated	Invitrogen	A-11034
Calcium Chloride	Invitrogen	K278001
2x HBS	Invitrogen	K278002
EDTA	Ambion	AM9261
BSA	Sigma	A7906
Blood Capillary Tubes	Fisher	22-260-950
Blood Collection Tube	Giene Bio-One	450480
Newborn Calf Serum	Atlanta biological	S11295
Erythropoiein	Amgen	5513-267-10
human SCF	Prospec	CYT-255
Human IL-3	Prospec	CYT-210
G-SCF	Prospec	CYT-220
GM-CSF	Prospec	CYT-221
MyeloCult (media for LTCIC assay)	Stem Cell Technologies	5100
Hydrocortisone Sodium Hemisuccinate	Stem Cell Technologies	7904
MEM alpha	Gibco	12561-056
1/2 cc Lo-Dose u-100 insulin syringe 28 G1/2	Becton Dickinson	329461
Mortor pestle	Coor tek	60316 and 60317
Isoflorane (Isothesia TM)	Butler Schien	29405
SOC	New England Biolabs	B90920s
Ampicillin	Sigma	A0166	100 mg/mL stock in water
Bacto agar (agar)	Difco	214050
Terrific broth	Becton Dickinson	243820
Agarose	Genemate	E-3119-500
Doxycycline chow	TestDiet.com	52662	modified RMH1500, Autoclavable 5LK8  with 0.0625% Doxycycline
Tamoxifen	Sigma	T5648
Iodonitrotetrazolium chloride	Sigma	I10406
Kits
Dneasy Blood & tissue kit	Qiagen	69506
GoTaq Green (taq polymerase with Green loadign dye)	Promega	M1722
miRNeasy Mini Kit  (RNA isolation kit)	Qiagen	217084
DNA Free Dnase Kit (DNAse treatment for RT PCR)	Ambion, Life Technologies	AM1906
Superscript III First Strand Synthesis (reverse transcriptase for cDNA synthesis)	Invitrogen	18080051
SYBR Green (taq polymerase mix with green interchalating dye for qPCR)	Bio-Rad	1725270
CD117 MicroBead Kit	Miltenyi	130-091-224
Human Long-Term Culture Initiating Cell Assay	Stemp Cell Technologies
Instruments
NAPCO series 8000 WJ CO2 incubator	Thermo scientific
Swing bucket rotor cetrifuge 5810R	Eppendorf
TC-10 automated cell counter	Bio-RAD
C-1000 Thermal cycler	Bio-RAD
Mastercycler Real Plex 2	Eppendorf
ChemiDoc Imaging System (imaging system for gels and western blots)	Bio-RAD	17001401
Hemavet (boold counter)	Drew-Scientific
LSR II (FACS analyzer)	BD
Fortessa I (FACS analyzer)	BD
FACSAriaII (FACS Sorter)	BD
Magnet Stand	Miltenyi
Irradiator	J.L. Shepherd and Associates, San Fernando CA	Mark I Model 68A	source Cs 137
Mice
ROSACreERT2	Jackson Laboratory
Scl-tTA	Dr. Claudia Huettner’s lab
BoyJ	mouse core facility at CCHMC
C57Bl/6	Jackson Laboratory
NSGS	mouse core facility at CCHMC
ROSACreERT2/c-Fosfl/fl Dusp1-/-	Made in house
ROSACreERT2/c-Fosfl/fl	Made in house
Cells
BaF3	Gift from George Daley, Harvard Medical School, Boston
WEHI	Gift from George Daley, Harvard Medical School, Boston
CML-CD34+ and Normal CD34+ cells	University Hospital, University of Cincinnati