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交配和四分体分离衣藻遗传分析
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JoVE Journal Biology
Mating and Tetrad Separation of Chlamydomonas reinhardtii for Genetic Analysis
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交配和四分体分离衣藻遗传分析

DOI:
10.3791/1274-v

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10:04 min

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August 12, 2009

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,
1Boyce Thompson Institute for Plant Research,Cornell University

Chapters

  • 00:05Title
  • 01:16Introduction
  • 02:00Preparing the Strains for Gametogenesis
  • 03:00Mating the Strains
  • 05:20Preparing for Tetrad Dissection
  • 07:56Dissecting the Tetrads
  • 09:00Representative Results: Tetrad Analysis
  • 09:20Conclusion

Summary

Automatic Translation

Automatic Translation

交配和四分体分离的遗传分析<em>衣藻</em>。在这里，我们展示了标准方法对配子，交配，合子萌发和四分体夹层。该协议包括一个易于遵循的一系列步骤，这将使科学家所不熟悉的遗传方法适合<em>衣</em>。

Tags

MatingTetrad SeparationChlamydomonas ReinhardtiiGenetic AnalysisCell BiologyGeneticsLife CycleGrowthManipulationGenomic ResourcesTransformabilityNucleusOrganellesVegetative DiploidsDominance AnalysisTetrad DissectionNuclear InheritanceOrganellar InheritanceAllelism TestEpistasy AnalysisMap-based CloningGametogenesisZygote Germination

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