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Quantificazione in situ di cellule beta pancreatiche Messa in topi
JoVE Journal
Biology
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JoVE Journal Biology
In situ Quantification of Pancreatic Beta-cell Mass in Mice
DOI:

09:50 min

June 07, 2010

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Chapters

  • 00:00Title
  • 00:57Introduction
  • 01:23Preparation of Specimens
  • 04:19Imaging and Quantifying the Beta-Cell Area in the Intact Pancreas
  • 07:58Slice Image Analysis of Beta Cells in an Intact Adult Pancreas
  • 09:27Conclusion

Summary

Automatic Translation

Il protocollo seguente delinea il processo di dissezione del pancreas per l'imaging fetta virtuale, e la successiva quantificazione di tutte le GFP-tagged beta-cellule del pancreas intero.

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