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1Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology,Rockefeller University, 2Berlin Institute for Medical Systems Biology,Max-Delbrück-Center for Molecular Medicine, 3Biozentrum der Universität Basel and Swiss Institute of Bioinformatics (SIB), 4Biozentrum der Universität Basel and Swiss Institute of Bioinformatics (SIB), 5Genomics Resource Center,Rockefeller University
Chapters
- 00:00Title
- 00:53Introduction
- 01:27In vivo UV Crosslinking
- 04:06Immunoprecipitation of Protein
- 07:07Recovery of Crosslinked RNA-Protein Segments
- 10:19Representative PAR-CLiP Results
- 12:01Conclusion
RNA-transkript är föremål för omfattande posttranscriptional reglering som förmedlas av en mängd gränsöverskridande agerar RNA-bindande proteiner (RBPs). Här presenterar vi en generaliserbar metod för att identifiera exakt och på ett transkriptom-omfattande RNA-bindning med RBPs.
Tags
PAR-CliPTranscriptome-wideBinding SitesRNA-binding ProteinsPost-transcriptional Gene RegulationRNA TranscriptsRNA-RBP InteractionsMicroRNA-containing Ribonucleoprotein ComplexesCell-type DependentlyInterplayHigh Resolution MapsIn Vivo Protein-RNA InteractionsGenetic ApproachesBiochemical ApproachesComputational ApproachesMicroarray ProfilingRIP-ChipKinetically Stable InteractionsRBP Recognition Element (RRE)Direct RBP Target Site InformationUV CrosslinkingImmunoprecipitationCLIPUV 254 Nm RNA-protein Crosslinking
