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生成的基因 C。线虫通过基因枪转化
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JoVE Journal Biology
Generation of Transgenic C. elegans by Biolistic Transformation
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生成的基因 C。线虫通过基因枪转化

DOI:
10.3791/2090-v

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10:15 min

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August 23, 2010

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, ,
1Department of Medicine,University of Pittsburgh

Chapters

  • 00:00Title
  • 00:31Introduction
  • 00:52Preparing Egg Plates
  • 02:44Seeding Egg Plates and Bombardment with DNA-coated Gold Particles
  • 07:58Worm Recovery After Bombardment
  • 08:42Identification of Transgenic Worms
  • 09:53Conclusion

Summary

Automatic Translation

Automatic Translation

转基因蠕虫常用<em> C。线虫</em>研究。描述的是一个简单而有效，协议引入蠕虫使用DNA包覆的金粒子枪法转基因。参与的努力和轰炸的结果相媲美注射代转基因动物。

Tags

Transgenic C. ElegansBiolistic TransformationNematode ModelWorm ResearchGene LocalizationFunction StudyTransgenic AnimalsMicroinjectionGermlineBiolistic BombardmentGold ParticlesBio-Rad PDS-1000 SystemMultiple Transgenic LinesExtrachromosomal ArraysIntegration

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