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从一个暗适应切片制备小鼠视网膜神经细胞的膜片钳录音
JoVE Journal
Neuroscience
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Neuroscience
Patch Clamp Recordings from Mouse Retinal Neurons in a Dark-adapted Slice Preparation
Please note that all translations are automatically generated.
Click here for the English version.
从一个暗适应切片制备小鼠视网膜神经细胞的膜片钳录音
DOI:
10.3791/2107-v
•
09:38 min
•
September 12, 2010
•
A. Cyrus Arman
,
Alapakkam P. Sampath
1
Neurosciences Graduate Program
,
University of Southern California
,
2
Zilkha Neurogenetic Institute, Department of Physiology and Biophysics
,
University of Southern California Keck School of Medicine
Chapters
00:00
Title
00:55
Introduction
01:08
Preparing Electrodes and Solutions
02:25
Setup of the Recording Apparatus
04:29
Eye Dissection and Slicing
07:42
Recording
08:44
Representative Results: Light-evoked from Retinal Neurons
09:08
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们描述了产生暗适应对小鼠视网膜电生理记录片的过程。
Tags
Patch Clamp Recordings
Mouse Retinal Neurons
Dark-adapted Slice Preparation
Visual Pigment
Retinal Photoreceptors
Intracellular Events
Cyclic-nucleotide-gated Channels
Membrane Potential
Neurotransmitter Release
Electrophysiological Recordings
Retinal Slice Preparations
Razor Blade
Filter Paper
Low Gelling Temperature Agar
Vibrating Microtome
Surface Damage
Light-evoked Responses
Infrared Light
Bleaching Visual Pigment
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