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生产 C。线虫</em通过重组工程与转基因 galK选择标记
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JoVE Journal Biology
The Production of C. elegans Transgenes via Recombineering with the galK Selectable Marker
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生产 C。线虫</em通过重组工程与转基因 galK选择标记

DOI:
10.3791/2331-v

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12:03 min

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January 11, 2011

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, , ,
1Department of Radiation Oncology,Beth Israel Deaconess Medical Center, Harvard Medical School, 2Department of Medicine, Division of Geriatric Medicine and Pittsburgh Institute for Neurodegenerative Diseases,University of Pittsburgh

Chapters

  • 00:05Title
  • 01:47Transfer Fosmid to SW016 Bacteria
  • 04:49Insertion of the galK Gene by Recombineering
  • 08:48Replacement of galK with Tag Sequences by Recombineering
  • 10:48Representative Results
  • 11:34Conclusion

Summary

Automatic Translation

Automatic Translation

能力生产的转基因<em>线虫</em>使用fosmids进行基因组DNA作为保留所有原生的调控元件，是特别有吸引力。描述的是一个简单和强大的过程，通过生产转基因的重组工程<em> galK</em>选择标记。

Tags

C. ElegansTransgenesRecombineeringGalK Selectable MarkerGFP Fusion ProteinsGene RegulationExpression PatternTandem Affinity Purification (TAP)PromoterReporter GeneCDNA3′ Untranslated RegionAlternative PromotersWorm Genomic DNAFosmid DNA ClonesE. Coli Based Recombineering ProcedureGFP InsertionTAP-tag InsertionPositive SelectionNegative Selection

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