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Preparation and Culture of Chicken Auditory Brainstem Slices
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JoVE Journal Neuroscience
Preparation and Culture of Chicken Auditory Brainstem Slices

Preparation and Culture of Chicken Auditory Brainstem Slices

DOI:
10.3791/2527-v

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11:16 min

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March 21, 2011

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, , ,
1Department of Otolaryngology-Head and Neck Surgery, Virginia Merrill Bloedel Hearing Research Center,University of Washington, 2Department of Physiology and Biophysics, Virginia Merrill Bloedel Hearing Research Center,University of Washington

Chapters

  • 00:00Title
  • 00:52Introduction
  • 01:28General Preparation for Dissection and Tissue Culture
  • 02:58Isolation of Chicken Embryo Auditory Brainstem
  • 04:40Preparation of the Brainstem for Slicing on the Vibratome
  • 05:10Slicing the Brainstem on the Vibratome
  • 08:14Storing Slices for in Vitro Physiology
  • 09:04Circuitry, Anatomy, and Physiological Properties of Cultured Brainstem Tissue
  • 10:43Conclusion

Summary

Automatic Translation

Automatic Translation

The chicken auditory brainstem is comprised of nuclei responsible for binaural sound processing. A single coronal slice preparation maintains the entire circuitry while the cultured approach provides a unique preparation to study the development of neuronal structure and auditory function at the molecular, cellular and network levels.

Tags

Chicken Auditory BrainstemModel SystemAnatomyPhysiologyAuditory ProcessingTemporal CodingExperimental ProceduresOrganotypic SlicesBinaural Sound ProcessingCircuitrySynaptic ActivityGene ExpressionNeural Circuit Development

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