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Journal / Biology / 漂白实验（FRAP和FLIP）来衡量生活胚胎干细胞的染色质的蛋白质动力学…
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漂白实验（FRAP和FLIP）来衡量生活胚胎干细胞的染色质的蛋白质动力学
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漂白实验（FRAP和FLIP）来衡量生活胚胎干细胞的染色质的蛋白质动力学

Article DOI: 10.3791/2696-v • 09:18 min • June 29th, 2011
June 29th, 2011 •
1, 1
1Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem

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我们描述漂白方法，包括荧光漂白后恢复（FRAP）和荧光漂白（翻转）监测胚胎干细胞（ES细胞）的染色质的蛋白质动力学的损失。染色质蛋白的动态，这被认为是研究染色质可塑性的手段之一，是增强多能干细胞。

Tags

发育生物学，第52期，实时成像，FRAP，翻转，胚胎干细胞（ES细胞），染色质，染色质的可塑性，蛋白质动力学
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