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多重PCR和反向线杂交检测(MPCR / RLB)
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)
Please note that all translations are automatically generated.
Click here for the English version.
多重PCR和反向线杂交检测(MPCR / RLB)
DOI:
10.3791/2781-v
•
10:15 min
•
August 06, 2011
•
Matthew V. N. O’Sullivan
,
Fei Zhou
,
Vitali Sintchenko
,
Fanrong Kong
,
Gwendolyn L. Gilbert
1
Centre for Infectious Diseases and Microbiology
,
University of Sydney
Chapters
00:05
Title
01:20
Preparation of Membrane
04:01
Hybridization and Detection
08:12
Representative mPCR/RLB Results
09:56
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
描述的是一种廉价的,同时检测到43个分子靶点的高通量方法。 MPCR / RLB的应用,包括微生物打字和多种病原体从临床标本的检测。
Tags
Multiplex PCR
Reverse Line Blot Hybridization Assay
Molecular Targets
Probe Hybridization
Nylon Membrane
Amine Modified Probes
Biotin Modified Primers
Streptavidin-peroxidase
Chemiluminescent Substrate
Photosensitive Film
Setup Costs
Consumable Costs
High Throughput
Short Turnaround Time
Sequence Variation Detection
Antibiotic Resistance Genes
Methicillin-resistant Staphylococcus Aureus Typing
Salmonella Sp Typing
Molecular Serotyping Of Streptococcus Pneumoniae And Streptococcus Agalactiae
Enterovirus Identification
Mycobacterium Sp Detection
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