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Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies
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Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies

Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies

DOI:
10.3791/1690-v

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08:19 min

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February 16, 2010

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, , , , , ,
1Department of Environment and Agro-Biotechnologies (EVA), Nutrition and Toxicology Unit (NuTox),Centre de Recherche Public-Gabriel Lippmann

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  • 00:05Title
  • 08:15Credits

Summary

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Protein extraction for proteomic analyses in fungal species requires high levels of standardization to be accomplished according with the minimum information about a proteomic experiment (MIAPE) guidelines. We present a video-protocol that includes a procedure for minimizing experimental bias during toxin induction and protein extraction from Fusarium spp.

Tags

Toxin InductionProtein ExtractionFusarium Spp.Proteomic StudiesMycotoxinsPathogenicity FactorsProtein ExpressionStandardizationBiological ReplicatesRobustness Of DataExtraction ProcedureCulture GrowthSolid Media

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