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Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies
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Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies

Article DOI: 10.3791/1690-v • 08:19 min • February 16th, 2010
February 16th, 2010 •
1, 1, 1, 1, 1, 1, 1
1Department of Environment and Agro-Biotechnologies (EVA), Nutrition and Toxicology Unit (NuTox), Centre de Recherche Public-Gabriel Lippmann

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Protein extraction for proteomic analyses in fungal species requires high levels of standardization to be accomplished according with the minimum information about a proteomic experiment (MIAPE) guidelines. We present a video-protocol that includes a procedure for minimizing experimental bias during toxin induction and protein extraction from Fusarium spp.

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Toxin Induction Protein Extraction Fusarium Spp. Proteomic Studies Mycotoxins Pathogenicity Factors Protein Expression Standardization Biological Replicates Robustness Of Data Extraction Procedure Culture Growth Solid Media
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